已发表论文

Fisetin 通过阻断 PAK4 信号通路来调控人口腔鳞状细胞癌的增殖

 

Authors Li Y, Jia S, Dai W

Received 30 August 2019

Accepted for publication 21 January 2020

Published 25 February 2020 Volume 2020:14 Pages 773—782

DOI https://doi.org/10.2147/DDDT.S229270

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 4

Editor who approved publication: Professor Manfred Ogris

Objective: Human oral squamous cell carcinoma (OSCC) is a major cause of mortality and morbidity worldwide. There is an urgent need to identify bioactive molecules and potential target genes that could inhibit carcinogenesis for OSCC therapy. Fisetin (3,7,3′,4′-tetrahydroxyflavone), a naturally occurring flavonoid, has been previously shown to have anti-proliferative activities in OSCC; however, its molecular mechanism is unknown.
Methods: Colony formation, cell viability, Boyden chamber, wound healing, and tumor xenograft assays were used to detect the impact of fisetin on OSCC cells in vitro and in vivo. Western blot analysis was used to examine the corresponding protein expression.
Results: Fisetin treatment significantly inhibited proliferation and promoted apoptosis by repressing PAK4 expression. Moreover, fisetin treatment attenuated cell migration by blocking PAK4 signaling pathways. In addition, the tumor xenograft showed anti-tumor growth effects of fisetin exposure in vivo.
Conclusion: Fisetin may represent a potential therapeutic strategy for human OSCC by targeting PAK4 signaling pathways.
Keywords: OSCC, fisetin, PAK4, PARP




Figure 3 Fisetin inhibits the cell cycle of PAK4-overexpressing OSCC cells.