已发表论文

在一例社区获得性肺炎病例中发现一种新型的高毒性鲍曼不动杆菌菌株

 

Authors Zhou H, Larkin PMK, Huang J, Yao Y, Zhu B, Yang Q, Hua X, Zhou J, Yang S, Yu Y

Received 28 December 2019

Accepted for publication 2 April 2020

Published 21 April 2020 Volume 2020:13 Pages 1147—1153

DOI https://doi.org/10.2147/IDR.S244044

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Joachim Wink

Purpose: Acinetobacter baumannii  is associated with both hospital-acquired infections and community-acquired pneumonia (CAP). Here, we describe a novel strain of A. baumannii  in a case of CAP in a previously healthy rural villager from Central Eastern China.
Materials and Methods: A. baumannii  isolated from the patient (LS01) was compared to well-characterized pathogenic strain (AB5075), nosocomial circulating strain in China (ZJ06), and wild-type strain (ATCC17978). Growth rate studies were conducted under different environmental stressors, and virulence studies were performed using Galleria mellonella  larvae. Whole genome sequencing (WGS) was performed using MinIon and MiSeq. Center for Genomic Epidemiology, CLCbio, Geneious, and Virulence Factors of Pathogenic Bacteria database were used for genomic analysis.
Results: LS01 grew significantly faster at 37°C and 42°C and in the presence of zinc compared to other strains. LS01 was more virulent in G. mellonella , killing all larvae within 8 h. Although WGS revealed 44 virulence genes, these genes were also present in the other strains. While two chromosomally encoded β-lactamases were identified, there were no plasmids identified and LS01 was pan-susceptible to all antibiotics tested. Phylogenetic analysis revealed that the closest related strains were only 72.552% identical, supporting a novel strain.
Conclusion: LS01 is a novel strain of hypervirulent yet pan-drug susceptible A. baumannii  isolated from a patient with no prior hospitalizations, sick contacts, or any of the typical risk factors. This raises concerns for an emerging pathogen, and more epidemiological studies should be conducted to assess the prevalence of this A. baumannii  strain.
Keywords: Acinetobacter baumannii , hypervirulent, community-acquired pneumonia, novel strain, emerging pathogen




Figure 1 Growth rate study...