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Authors Luo C, Ke YT, Yuan YY, Zhao M, Wang FY, Zhang YS, Bu SH
Received 15 July 2016
Accepted for publication 20 September 2016
Published 29 November 2016 Volume 2016:12 Pages 3051—3059
DOI https://doi.org/10.2147/NDT.S117337
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Prof. Dr. Roumen Kirov
Peer reviewer comments 3
Editor who approved publication: Professor Wai Kwong Tang
Background: Radix Puerariae and hawthorn fruit have been demonstrated to treat
diabetes. They offer potential benefits for preventing depression in diabetes.
Objective: The aim of this study was to investigate whether the combination of
Radix Puerariae and hawthorn fruit (CRPHF) could prevent depression in a
diabetic rat model generated by feeding the rats with a high-fat diet and a
low-dose streptozotocin (STZ).
Methods: The CRPHF was provided by the Shanghai Chinese Traditional Medical
University. Twenty-four rats were randomly divided into four groups: normal
control, normal-given-CRPHF (NC), diabetic control, and diabetic-given-CRPHF
(DC) groups. The type 2 diabetic model was created by feeding the rats with a
high-fat diet for 4 weeks followed by injection of 25 mg/kg STZ. CRPHF was
given at 2 g/kg/d to the rats of NC and DC groups by intragastric gavage daily
for 4 weeks after the type 2 diabetic model was successfully created. Body
weight, random blood glucose (RBG), oral glucose tolerance test, total
cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol
(HDL-C), and low-density lipoprotein cholesterol (LDL-C) were measured during
the study. Depressive-like behavior was evaluated at the end of the treatment
by using the open field test (OFT), the elevated plus-maze test (EPMT),
locomotor activity test (LAT), and forced swimming test (FST). Levels of
extracellular signal-regulated protein kinase (ERK) and brain-derived neurotrophic
factor (BDNF) in the prefrontal cortex were evaluated by using Western blot.
Results: 1) CRPHF reduced RBG and improved glucose tolerance in diabetic rats; 2)
CRPHF reduced TC and TG but did not significantly change HDL-C or LDL-C in
diabetic rats; 3) CRPHF reversed the loss in body weights observed in diabetic
rats; 4) CRPHF reduced depressive-like behavior as measured by OFT, EPMT, LAT,
and FST; 5) BDNF was upregulated, and ERK was activated in the prefrontal
cortex of diabetic rats treated with CRPHF.
Conclusion: CRPHF has the potential of preventing depression in patients with
diabetes.
Keywords: Puerarin, Crataegus, diabetes mellitus, depression, BDNF