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Authors Feng Y, Liu WC, Pan LZ, Jiang C, Zhang CX, Lu YX, Nie ZY, Jin LJ
Received 31 March 2017
Accepted for publication 31 May 2017
Published 28 June 2017 Volume 2017:11 Pages 1927—1939
DOI https://doi.org/10.2147/DDDT.S138489
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Akshita Wason
Peer reviewer comments 2
Editor who approved publication: Dr Tuo Deng
Abstract: Four botulinumtoxin type A
(BoNT/A) products, onabotulinumtoxinA (A/Ona), incobotulinumtoxinA (A/Inco),
lanbotulinumtoxinA (A/Lan) and chinbotulinumtoxinA (A/Chin), are applied in the
present study, among which A/Chin is newly produced. We aimed to compare the
neurotoxic potency of these toxins by the gauge of muscle strength reduction.
Furthermore, potential molecular and cellular mechanisms were also explored.
According to our data, muscle strengths in the four toxin groups were all
significantly decreased after injection for 1 week. A/Chin achieved the
most obvious reduction in muscle strength as compared to the other three
products at the dose of 0.5 U. However, there was no difference between
the four toxins when increased to 2 U. As the toxins wore off, muscle
strength recovered to basal level 12 weeks postinjection. We further measured
the expression levels of key factors involved in neuromuscular junction
stabilization and muscle genesis. Our results showed that nicotinic
acetylcholine receptor, myogenic regulatory factors and muscle-specific
receptor tyrosine kinase were all significantly upregulated upon BoNT/A
treatment. Consistent with the result of muscle strength, A/Chin had the most
obvious induction of gene expression. Moreover, we also found local
inflammation response following BoNT/A injection. Owing to lack of complexing
proteins, both A/Inco and A/Chin stimulated relatively lighter inflammation
compared to that of A/Ona and A/Lan groups. In conclusion, our study provided
evidence for the efficacy of the novel A/Chin and its similar functional mode
to that of A/Ona, A/Inco and A/Lan. In addition, A/Chin has superiority in
inducing muscle paralysis and inflammation stimulation, which may indicate
faster onset and longer duration of this novel A/Chin.
Keywords: A/Chin, A/Lan,
A/Ona, A/Inco, neurotoxic potency