已发表论文

结直肠癌患者中 PAX1/SOX1  的 DNA 高甲基化状态和基因表达

 

Authors Huang J, Tan ZR, Yu J, Li H, Lv Q, Shao Y, Zhou H

Received 6 June 2017

Accepted for publication 14 July 2017

Published 26 September 2017 Volume 2017:10 Pages 4739—4751

DOI https://doi.org/10.2147/OTT.S143389

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Ashok Kumar Pandurangan

Peer reviewer comments 2

Editor who approved publication: Dr Carlos Vigil Gonzales

Background: Colorectal cancer (CRC) is a widespread and aggressive carcinoma with poor prognosis. Hypermethylation of specific gene promoters is an important mechanism of CRC. In this study, we investigated the hypermethylation of paired boxed gene 1 (PAX1 ) and sex-determining region Y-related high-mobility group box 1 (SOX1 ) genes in CRC tissues.
Methods: DNA methylation at cg2,09,07,471 PAX1  and cg0,66,75,478 SOX1  from 166 cancer tissues and 37 normal tissues from CRC patients were compared using datasets downloaded from The Cancer Genome Atlas. Quantitative methylation-specific polymerase chain reaction and assay of PAX1  and SOX1  were performed in dissected tumor and paracancerous tissues by surgery from 41 CRC patients. Quantitative reverse transcription polymerase chain reaction and immunohistochemistry assay were performed in both CRC and paired normal tissues to detect mRNA and protein expression, respectively.
Results: Methylation levels of PAX1/SOX1  genes were significantly higher in cancer tissues than in paired normal tissues. PAX1  and SOX1  genes were methylated in 28 (68.3%) of the 41 CRC samples but in 5 (12.2%) and 0 (0%) of the paired normal control samples (both <0.001), respectively. Sensitivities and specificities of PAX1  methylation for the detection of cancer were 68.3% and 87.8%, respectively, whereas the corresponding values for SOX1  were 68.3% and 100%. However, the Kaplan–Meier analysis illustrated no significant difference in the overall survivals between patients with high and low methylation levels of SOX1  or PAX1  (>0.5). In addition, the methylation level of PAX1/SOX1  was significantly higher in CRC patients with high TNM stage (TNM stage III/IV, 3.11±2.43) than those with low TNM stage (TNM stage I/II, 1.26±2.94, <0.05). Relative RNA and protein expression levels of PAX1/SOX1  were both significantly lower in CRC tissues than in their paired normal tissue.
Conclusions: This study is the first analysis of the methylation of PAX1/SOX1 , which may be new biomarkers for CRC screening.
Keywords: colorectal cancer, PAX1 SOX1 , molecular biomarker, DNA methylation, epigenetic