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Authors Wu C, Xu J, Hao Y, Zhao Y, Qiu Y, Jiang J, Yu T, Ji P, Liu Y
Received 3 May 2017
Accepted for publication 25 September 2017
Published 31 October 2017 Volume 2017:12 Pages 7979—7992
DOI https://doi.org/10.2147/IJN.S140957
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Thiruganesh Ramasamy
Peer reviewer comments 3
Editor who approved publication: Dr Linlin Sun
Abstract: In this study, we
developed a lipid-coated hollow calcium phosphate (LCP) nanoparticle for the
combined application of two chemotherapeutic drugs to human lung cancer A549
cells. Hydrophilic doxorubicin (DOX) was incorporated into the hollow structure
of hollow calcium phosphate (HCP), and a lipid bilayer containing hydrophobic
paclitaxel (PTX) was subsequently coated on the surface of HCP. The study on
combinational effects demonstrated that the combination of DOX and PTX at a
mass ratio of 12:1 showed a synergistic effect against A549 cells. The particle
size, zeta potential, and encapsulation efficiency were measured to obtain
optimal values: particle size was 335.0 3.2 nm, zeta potential -41.1 mV, and
encapsulation efficiency 80.40%±2.24%. An in vitro release study indicated that
LCP produced a sustained drug release. A549 cells had a better uptake of LCP
with good biocompatibility. Furthermore, in vitro cytotoxicity experiment,
apoptosis analysis, in vivo anti-tumor efficacy and protein expression analysis
of Bax, Bcl-2, and Caspase-3 demonstrated that the co-delivery system based on
LCP had significant synergistic anti-tumor activity. All conclusions suggested
that LCP is a promising platform for co-delivery of multiple anti-tumor drugs.
Keywords: doxorubicin,
paclitaxel, co-delivery, lipid, hollow calcium phosphate, lung cancer cell