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已发表论文
骨髓间充质干细胞移植促进创伤性脑损伤小鼠的血管新生并改善神经功能
Authors Guo SW, Zhen YW, Wang AR
Received 10 May 2017
Accepted for publication 14 June 2017
Published 6 November 2017 Volume 2017:13 Pages 2757—2765
DOI https://doi.org/10.2147/NDT.S141534
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Prof. Dr. Roumen Kirov
Peer reviewer comments 2
Editor who approved publication: Professor Wai Kwong Tang
Abstract: Traumatic
brain injury (TBI) has emerged as a leading cause of mortality and morbidity
worldwide. Transplantation of bone mesenchymal stem cells (BMSCs) has emerged
as a promising treatment for various central nervous system diseases. This
study aims to evaluate the effect of BMSCs transplantation by intravenous
injection on neurological function and angiogenesis of the TBI mice. C57BL/6
male mice were randomly divided into four groups: control, sham, TBI, and BMSC.
Functional neurological evaluation was performed 1, 4, 7, 14, and 21 days after
TBI using neurological severity scores. The impairment of learning and memory
in mice was evaluated 14 days after TBI by Morris water maze experiment. Mice
were sacrificed 14 days after TBI, and then brain sections were stained by
terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick
end labeling staining to assess brain neuronal apoptosis. Immunohistochemistry
was conducted to evaluate caspase-3 activity and identify vascular distribution
and microvessel density. Protein and mRNA
levels of vascular endothelial growth factor (VEGF) and angiogenin-1 (Ang-1) in
brain tissues were analyzed by Western blot and quantitative real-time
polymerase chain reaction, respectively. BMSCs transplantation promoted
recovery of neurological function, ameliorated impairment of learning and
memory, attenuated neuronal apoptosis, and diminished caspase-3 activation in
mice after TBI. Also, BMSCs transplantation upregulated expressions of VEGF and
Ang-1 and promoted the formation of microvessels in brain tissues after TBI.
Our study demonstrated the important role of BMSCs transplantation in TBI mouse
and indicated that the underlying mechanism was through promoting angiogenesis
and improving neurological function. This provides a novel and effective
strategy for cell-based therapy in the treatment of TBI.
Keywords: TBI, BMSCs, angiogenesis, neurological function
Keywords: TBI, BMSCs, angiogenesis, neurological function
