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已发表论文

从华东地区分离的流行耐甲氧西林金黄色葡萄球菌中的 sasX pvl  qacA/B  基因的分布

 

Authors Kong H, Fang L, Jiang R, Tong J

Received 6 October 2017

Accepted for publication 28 November 2017

Published 9 January 2018 Volume 2018:11 Pages 55—59

DOI https://doi.org/10.2147/IDR.S153399

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 2

Editor who approved publication: Dr Joachim Wink

Background: Methicillin-resistant Staphylococcus aureus  (MRSA) is a major nosocomial pathogen. Various virulence and antiseptic-resistant factors increase the pathogenicity of MRSA strains and allow for increased infection rates.
Purpose: The purpose of this study was to investigate the prevalence and distribution of virulence-associated and antiseptic-resistant genes from epidemic MRSA strains isolated from East China.
Methods: A newly designed multiplex PCR assay was used to assess whether the virulence-associated genes sasX  and pvl  and the chlorhexidine tolerance gene qacA/B  were present in 189 clinical isolates of MRSA. Multilocus sequence typing (MLST) and Staphylococcal protein A (spa ) typing of these isolates were also performed. The frequency of these genes in isolates with epidemic sequence types (STs) was investigated. 
Results: Twenty STs and 36 spa  types with five epidemic clones (ST5-t311, ST59-t437, ST5-t002, ST239-t030, and ST239-t037) were identified. The prevalence of sasX pvl , and qacA/B  in all isolates was 5.8%, 10.1%, and 20.1%, respectively. The prevalences of these genes in isolates with ST5, ST59, ST239, and other ST genetic backgrounds were all significantly different (<0.001). Isolates that had the highest frequency of sasX pvl , or qacA/B  were ST239 (33.3%), ST59 (28.9%), and ST5 (34.1%), respectively. The gene distribution pattern from all of the isolates showed that sasX pvl qacA/B +, sasX pvl +qacA/B –, and sasX +pvl qacA/B – were closely associated with epidemic clones ST5-t311, ST59-t437, and ST239-t037, respectively.
Conclusion: There are significant differences in the prevalence of virulence-associated and antiseptic-resistant genes in epidemic MRSA strains. Using this information, more effective control and prevention strategies for nosocomial MRSA infections can be developed.
Keywords: MRSA, MLST, virulence genes, sasX pvl qacA/B , multiplex PCR