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Authors Liu PF, Yan P, Zhao DH, Shi WF, Meng S, Liu Y, Liu B, Li GF, Xie LX
Received 13 November 2017
Accepted for publication 15 December 2017
Published 28 February 2018 Volume 2018:13 Pages 741—751
DOI https://doi.org/10.2147/COPD.S156865
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Charles Downs
Peer reviewer comments 2
Editor who approved publication: Prof. Dr. Chunxue Bai
Objective: The objective of the study was to analyze the effect of environmental
factors on the differential expression of microRNAs in the peripheral blood of
migratory and local patients in northern People’s Republic of China and on
clinical symptoms of local patients in northern People’s Republic of China with
COPD.
Methods: A total of 118 patients in the northern region and 8 migratory patients
were enrolled in this prospective study. We collected general information.
Blood samples were collected from 9 patients in the Beijing group, from 8
patients in the migratory group and from 9 healthy control subjects. After
extracting the total RNA from these 3 groups, serum miRNA was identified by
Solexa sequencing. We collected COPD assessment test (CAT) and Modified British
Medical Research Council (mMRC) scores at different levels of air pollution and
also collected the number of exacerbations over the year prior to the baseline
and in the year preceding the follow-up.
Results: In total 9 miRNAs were differentially expressed. When air quality
index (AQI) >100, the CAT and mMRC scores at baseline were significantly
higher than those when the AQI ≤100 (P <0.001). When
AQI >100, the follow-up CAT and mMRC scores were significantly higher than
those when AQI ≤100 (P <0.001).
Follow-up mMRC scores were significantly higher than baseline scores (P =0.04). When AQI ≤100, the
baseline CAT score of the group with fewer symptoms was 6.50 (4.00–8.75).
However, when AQI >100, the baseline CAT score of this fewer symptoms group
was 10.00 (6.25–12.00). The median CAT score was close to 10. When
AQI ≤100, the follow-up CAT score of the fewer symptoms group was 8.00
(4.25–12.00). However, when AQI >100, the follow-up CAT score of the
fewer symptoms group was 9.50 (6.00–16.75). The median CAT score was close to
10.
Conclusion: Environmental factors may cause differential expression of miRNAs
in the peripheral blood of migratory and local patients in northern People’s
Republic of China. Air pollution may aggravate clinical symptoms of patients
with COPD.
Keywords: COPD, miRNA, environment, CAT score, mMRC