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Authors Zhang ZX, Zhang WN, Sun YY, Li YH, Xu ZM, Fu WN
Received 9 November 2017
Accepted for publication 10 January 2018
Published 8 March 2018 Volume 2018:11 Pages 1323—1331
DOI https://doi.org/10.2147/OTT.S156582
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Amy Norman
Peer reviewer comments 2
Editor who approved publication: Dr Tohru Yamada
Purpose: CREB,
MYCY1 and NAT10 are involved in cancer cell migration. However, the
relationship between these three proteins and their role in laryngeal cancer
cell migration remains unknown.
Methods: Transient gene transfection was performed in laryngeal cancer
cells. Bioinformatics analysis was used to predict the binding of CREB to MYCT1
promoter. Binding of CREB to the promoter of MYCT1 was monitored by luciferase
reporter assay and chromatin immunoprecipitation method in vitro and in vivo,
respectively. Real-time RT-PCR and Western bolt were applied to detect gene
transcription and translation levels, respectively. Laryngeal cancer cell
migration was assayed by transwell chamber experiment.
Results: CREB protein expression was significantly up-regulated in laryngeal
cancer tissues and associated with cancer differentiation, tumor stage, and
lymphatic metastasis. CREB inhibits MYCT1 expression by direct binding to its
promoter. Meanwhile, MYCT1 has a negative impact on the NAT10 gene expression.
Furthermore, CREB promotes NAT10 expression via down-regulating the MYCT1 gene
expression. In addition, contrary to MYCT1, CREB and NAT10 enhanced laryngeal
cancer cell migration. MYCT1 and NAT10 significantly rescued the effects of
CREB and MYCT1 on Hep2 cell migration, respectively.
Conclusion: CREB promotes laryngeal cancer cell migration via MYCT1/NAT10
axis, suggesting that CREB might be a potential prognostic marker in laryngeal
cancer.
Keywords: laryngeal cancer, CREB, MYCT1, NAT10, migration