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已发表论文

氧化锌纳米颗粒通过激活 PINK1/Parkin 介导的线粒体自噬可在 CAL 27 口腔癌细胞系中诱导毒性

 

Authors Wang J, Gao S, Wang S, Xu Z, Wei L

Received 16 February 2018

Accepted for publication 26 April 2018

Published 20 June 2018 Volume 2018:13 Pages 3441—3450

DOI https://doi.org/10.2147/IJN.S165699

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Alexander Kharlamov

Peer reviewer comments 3

Editor who approved publication: Dr Linlin Sun

Background: Tongue squamous cell carcinoma (tongue cancer) is one of the most common malignancies in the oral maxillofacial region. The tumor easily relapses after surgery, and the prognosis remains poor. Recently, zinc oxide nanoparticles (ZnO NPs) were shown to target multiple cancer cell types. In this study, we aimed to elucidate the anticancer effect of ZnO NPs on CAL 27 human tongue cancer cells and identify the role of PINK1/Parkin-mediated mitophagy in this effect.
Materials and methods: We analyzed the dose-dependent cytotoxic effects of ZnO NPs on CAL 27 cells. Cells were cultured in media containing 0, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100 µg/mL ZnO NPs for 24 h. We further examined the intracellular reactive oxygen species levels, monodansylcadaverine intensity and mitochondrial membrane potential following the administration of 25 µg/mL ZnO NPs for 4, 8, 12, or 24 h and investigated the role of PINK1/Parkin-mediated mitophagy in ZnO NP-induced toxicity in CAL 27 cells.
Results: The viability of CAL 27 cells decreased after treatment with increasing ZnO NP concentrations. The inhibitory concentration 50% of the ZnO NPs was calculated as 25 µg/mL. The ZnO NPs increased the intracellular reactive oxygen species levels and decreased the mitochondrial membrane potential in a time-dependent manner as well as activated the PINK1/Parkin-mediated mitophagy process in CAL 27 cells.
Conclusion: Based on our findings, ZnO NPs may possess potential anticancer activity toward tongue cancer cells.
Keywords: zinc oxide nanoparticles, mitophagy, tongue cancer; anticancer therapy




Figure 2 (A) Effects of various concentrations of ZnO NPs on the cell...