已发表论文

c-jun  的沉默减少了放射抗性人鼻咽癌细胞系 CNE-2R 的细胞迁移、侵袭和 EMT

 

Authors Lin G, Yu B, Liang Z, Li L, Qu S, Chen K, Zhou L, Lu Q, Sun Y, Zhu X

Received 16 January 2018

Accepted for publication 9 May 2018

Published 4 July 2018 Volume 2018:11 Pages 3805—3815

DOI https://doi.org/10.2147/OTT.S162700

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Andrew Yee

Peer reviewer comments 4

Editor who approved publication: Dr William Cho

Background: Previously, we found that c-jun  was highly expressed in radiation-resistant human nasopharyngeal carcinoma cells (CNE-2R) compared with human nasopharyngeal carcinoma cells (CNE-2). 
Materials and methods: In this study, we first used the scratch assays and transwell assays to detect the migration and invasion of CNE-2R and CNE-2 cells and tested the epithelial mesenchymal transformation (EMT)-related proteins E-cadherin and N-cadherin by Western blot analysis. Subsequently, c-jun  was knocked down to establish the effect of c-jun  on EMT, migration, and invasion of CNE-2R cells both in vitro and in vivo. 
Results: A high EMT level, CNE-2R cells were more capable of migration and invasion than CNE-2 cells. Moreover, silencing of c-jun  has upregulated the expression of E-cadherin and downregulated N-cadherin in CNE-2R cells, and subsequently the migration and invasion capacity of the cells was decreased. Consistent with in vitro results, in vivo studies indicated that the c-jun  silencing reduced pulmonary migration of CNE-2R cells. Immunohistochemistry of lung metastatic tumor tissue showed that E-cadherin was upregulated, and N-cadherin was downregulated. 
Conclusion: These findings suggest that silencing of c-jun  in CNE-2R cells reduces cells migration, invasion, and EMT both in vitro and in vivo.
Keywords: nasopharyngeal carcinoma, c-jun , migration, invasion, EMT




Figure 2 Effective shRNA-mediated knockdown of c-jun in the CNE-2R cells.