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已发表论文

lncRNA ANRIL  的下调抑制人骨肉瘤细胞的生长和转移

 

Authors Guan H, Mei Y, Mi Y, Li C, Sun X, Zhao X, Liu J, Cao W, Li Y, Wang Y

Received 6 April 2018

Accepted for publication 25 May 2018

Published 14 August 2018 Volume 2018:11 Pages 4893—4899

DOI https://doi.org/10.2147/OTT.S170293

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 3

Editor who approved publication: Dr Carlos E Vigil

Background: This study was designed to research the potential function of lncRNA ANRIL  in osteosarcoma (OS).
Materials and methods: Quantitative real-time PCR, cell counting kit-8, wound healing assay, Transwell assay, flow cytometric analysis, caspase activity analysis, and Western blot were carried out.
Results: ANRIL  was remarkably upregulated in human OS tissues and cells, and knockdown of ANRIL  significantly suppressed MG63 cell proliferation, migration, and invasion and promoted apoptosis. Moreover, our mechanistic research findings verified that ANRIL -influenced growth and apoptosis may be partly through regulation of caspase-3 and Bcl-2. Migration and invasion were influenced via ANRIL -mediated regulation of MTA1, TIMP-2, and E-cadherin.
Conclusion: Our finding demonstrates that ANRIL  plays vital roles in OS growth and metastasis.
Keywords: osteosarcoma, ANRIL , proliferation, invasion, apoptosis, long noncoding RNA




Figure 1 lncRNA ANRIL expression in OS tissues and cell lines.