已发表论文

骨桥蛋白可促进癌细胞耐药、侵袭和乳酸生成,并与晚期非小细胞肺癌患者预后不良有关

 

Authors Ouyang XP, Huang Y, Jin X, Zhao W, Hu T, Wu F, Huang J

Received 28 January 2018

Accepted for publication 20 July 2018

Published 19 September 2018 Volume 2018:11 Pages 5933—5941

DOI https://doi.org/10.2147/OTT.S164007

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Narasimha Reddy Parine

Peer reviewer comments 3

Editor who approved publication: Dr Samir Farghaly

Background: Osteopontin (OPN), a member of the small integrin binding ligand N-linked glycoprotein family, has been analyzed in numerous types of human malignancy.
Purpose: The present study detected the expression levels of OPN and evaluated its role in tumor progression in patients with non-small cell lung cancer (NSCLC). 
Patients and methods: OPN expression levels were detected using immunohistochemistry in 101 NSCLC tumors. The mRNA and protein levels have significant difference between advanced NSCLC and stage I/II NSCLC. The drug resistance, invasive ability and lactate production of NSCLC cancer cell lines (A549 and SK-MES-1) were detected in cancer cells with the disturbance of OPN. 
Results: Immunostaining indicated that OPN was primarily expressed in the cytoplasm of NSCLC cells. Moreover, OPN correlates with NSCLC clinical traits. The results demonstrated that OPN expression levels significantly correlated with cancer differentiation, distant metastasis and the efficacy of platinum-based treatment. Notably, the results identified OPN expression levels as a potential factor for predicting the response of cells to first-line platinum-based chemotherapy using multivariate analysis, as well as predicting cancer differentiation and distant metastasis. Additionally, the abrogation of OPN levels reduced lactate production in NSCLC cells and occurred along side with the downregulation of lactate dehydrogenase A (LDHA). 
Conclusion: The results of the current study suggest that OPN may be able to predict poor prognosis and cisplatin resistance in patients.
Keywords: osteopontin, non-small-cell lung cancer, clinical outcome, drug resistance, invasion, lactate production




Figure 1 Expression levels of OPN in NSCLC tissue.