已发表论文

中国乳腺癌患者分子亚型中 CD4+ and CD8+ 耗竭肿瘤浸润淋巴细胞的分布

 

Authors Shi F, Chang H, Zhou Q, Zhao YJ, Wu GJ, Song QK

Received 13 March 2018

Accepted for publication 12 August 2018

Published 21 September 2018 Volume 2018:11 Pages 6139—6145

DOI https://doi.org/10.2147/OTT.S168057

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Geoffrey Pietersz

Purpose: Breast cancer (BC) is the leading cancer affecting Chinese women; however, the immune microenvironment between molecular subtypes is less reported. This study aimed to investigate the distribution of tumor-infiltrating lymphocyte (TIL) subpopulations, especially exhausted CD4+ and CD8+ TILs in Chinese BC patients.
Patients and methods: A total of 133 patients with breast invasive ductal carcinoma were recruited consecutively from January 1, 2012 to December 31, 2013, and TILs were detected in H&E-stained sections. Expression profiling of PD-1, CD4, and CD8 was determined by immunohistochemistry on 4 µm formalin-fixed paraffin-embedded tissue sections. The distribution of TILs was analyzed based on hormone receptor status and molecular subtypes.
Results: PD-1+, CD4+, and CD8+ TILs distributed differently based on molecular subtypes. Compared to Luminal A, triple-negative breast cancer (TNBC) patients had more PD-1+ TILs (39/high-power field [HPF] vs 11/HPF), PD-1+ helper T (CD4+) cells (28/HPF vs 10/HPF), and PD-1+ cytotoxic (CD8+) T-cells (3/HPF vs 2/HPF).
Conclusion: TILs are distributed differently based on molecular subtypes. TNBC patients exhibit more PD-1+ exhausted TILs, representing an inhibitory immune microenvironment. PD-1/PD-L1 pathway is a potential therapeutic target of TNBC.
Keywords: breast cancer, tumor-infiltrating lymphocyte, PD-1, molecular subtype




Figure 2 Coexpression of CD4/PD-1 and CD8/PD-1 on TILs in Luminal A and TNBC (×400 magnification).