Purpose: The long noncoding RNA RGMB-AS1  plays an important part in the genesis and progression of multiple human cancers. Nonetheless, little is known regarding its expression, roles, and mechanisms of action in gastric cancer (GC). This study was aimed at investigating the relationship between RGMB-AS1  and GC and illustrating the mechanisms of action of RGMB-AS1  therein.
Methods: RGMB-AS1  expression in GC was measured via reverse-transcription quantitative PCR. A series of experiments including Cell Counting Kit-8 assay, flow-cytometric analysis of apoptosis, Transwell migration and invasion assays, and in vivo tumorigenesis experiment were conducted to test the effects of RGMB-AS1  on the malignant phenotype of GC cells. The molecular events behind the oncogenic actions of RGMB-AS1  in GC were elucidated through subcellular fractionation, RNA immunoprecipitation assay, bioinformatics analysis and luciferase reporter assay.
Results: RGMB-AS1  upregulation was confirmed in GC tissues and cell lines. Higher RGMB-AS1  expression was associated with adverse clinical parameters and negatively correlated with patient overall survival. RGMB-AS1  knockdown inhibited GC cell proliferation, facilitated apoptosis, and reduced migration and invasion in vitro. Further experiments revealed that RGMB-AS1  knockdown decreased the tumor growth of GC cells in vivo. Mechanistically, RGMB-AS1  functioned as a competing endogenous RNA upregulating histone deacetylase 4 (HDAC4) by sponging microRNA-574 (miR-574). Rescue experiments indicated that miR-574 inhibition and HDAC4 reintroduction reversed the effects of the RGMB-AS1  knockdown on GC cells.
Conclusion: The RGMB-AS1 –miR-574–HDAC4 regulatory network contributes to the malignancy of GC, thereby offering a novel target for the diagnosis, prognosis, and/or treatment of GC.
Keywords: tumor therapy, gastric cancer, miR-574, RGMB-AS1 , histone deacetylase 4