Purpose: To characterize the genetic feature of the carbapenems resistant Acinetobacter johnsonii  strain Acsw19 isolated from municipal sludge. This strain was found to carry two copies of bla _NDM-1, cmlB1 -like gene, and bla _OXA-211-like gene along with other 8 antimicrobial resistance genes, 3 plasmids, 15 genomic islands and 8 prophages.
Methods: A carbapenem-resistant Acinetobacter johnsonii  strain Acsw19 isolated from municipal sludge was subjected to whole-genome sequencing (WGS) via the PacBio and Illumina MiSeq platforms. Thereafter, the characteristic was analyzed by a series of bioinformatics software.
Results: The results showed that the genome of Acsw19 was consisted of a 3,433,749 bp circular chromosome and 3 circular plasmids, pAcsw19-1 (11,161 bp), pAcsw19-2 (351,885 bp) and pAcsw19-3 (38,391bp), respectively. Resistome analysis showed that Acsw19 carried 12 antimicrobial resistance genes, including 6 [cmlB1 -like, bla _NDM-1, bla _OXA-58, aph  ( 3ʹ )-VIa, msr(E)  and mph(E) ] in the plasmid pAcsw19-2 and 6 (bla _OXA-211-like, bla _NDM-1, aph(3")-Ib, aph(6)-Id, sul2 , and floR ) in the chromosome genome. Specifically, the cmlB1 -like gene shared 86.33%, 71.7% and 71.9% similarities with the cmlB1, cmlA4  and cmlA8  gene, and the bla _OXA-211-like gene shared 94.4%, 95.39% and 96.36% similarities with bla _OXA-211, bla _OXA-643 and bla _OXA-652, at the nucleotide level, respectively. Phylogenetic analysis showed that the bla _OXA-211-like gene and cmlB1 -like gene had the closest evolutionary relationship with bla _OXA-643 and cmlB1 , respectively. These results indicated that the bla _OXA-211-like and cmlB1 -like genes identified in the current study should be the novel variant resistance genes.
Conclusion: Carrying of two copies of bla _NDM-1, cmlB1 -like, bla _OXA-211-like and along with other 8 antimicrobial resistance genes, 3 plasmids, 15 genomic islands and 8 prophages Acinetobacter johnsonii  strain might increase the possibility of spreading of resistance genes.
Keywords: Acinetobacter johnsonii , bla _NDM-1, bla _OXA, genomic island