Purpose: Lanthanum oxide (La₂O₃) nanoparticles (NPs) have been widely used in catalytic and photoelectric applications, but the reproductive toxicity is still unclear. This study evaluated the reproductive toxicity of two different-sized La₂O₃ particles in the testes.
Materials and Methods: Fifty Kunming mice were randomly divided into five groups. Mice were treated with La₂O₃ NPs by repeated intragastric administration for 90 days (control, nano-sized with 5, 10, 50 mg/kg BW and micro-sized with 50 mg/kg BW). Mice in the control group were treated with de-ionised water without La₂O₃ NPs. Sperm parameters, testicular histopathology, TEM assessment, hormone assay and nuclear factor erythroid 2-related factor 2 (Nrf-2) pathway were performed and evaluated.
Results: The body weight of mice treated with La₂O₃ NPs or not had no difference; sperm parameters and histological assessment showed that La₂O₃ NPs could induce reproductive toxicity in the testicle. Serum testosterone and gonadotropin-releasing hormone (GnRH) in the NH (nano-sized with 50 mg/kg BW) group were markedly decreased relative to control group, and an increase of luteinizing hormone (LH) in NH group was detected. Additionally, transmission electron microscopy revealed that the ultrastructural abnormalities induced by La₂O₃ NPs were more severe than La₂O₃ MPs in the testes. Furthermore, La₂O₃ NPs treatment inhibited the translocation of nuclear factor erythroid 2-related factor 2 (Nrf-2) from the cytoplasm into the nucleus as well as the expression of downstream genes NAD(P)H quinone oxidoreductase1 (NQO1), hemeoxygenase 1 (HO-1) and (glutathione peroxidase) GSH-Px, thus abrogating Nrf-2-mediated defense mechanisms against oxidative stress.
Conclusions: The results of this study demonstrated that La₂O₃ NPs improved the spermatogenesis defects in mice. La₂O₃ NPs inhibited Nrf-2/ARE signaling pathway that resulted in apoptosis in the mice testes.
Keywords: La₂O₃ nanoparticles, reproductive toxicity, inflammation, Nrf-2/ARE signaling pathway, apoptosis