Background: RmtF, as 16S rRNA methyltransferase, leads to high-level resistance to aminoglycoside and is now barely reported.
Methods and Results: Three rmtF -positive Klebsiella pneumoniae  isolates, belonging to the pandemic clone sequence type 15, were isolated from children and coproduced bla _OXA-232 and bla _CTX-M-15. The rmtF  gene was located on an IncFIB transformable plasmid of 128,536-bp and bla _OXA-232 was on a 6141-bp ColKP3 plasmid, respectively.
Conclusion: Plasmids with rmtF  found worldwide, shared relatively low similarity, and merely matched partly in their multidrug resistance region. Notably, clinical isolates coproducing rmtF  and bla _OXA-232 are gradually increasing in China.
Keywords: Klebsiella pneumoniae , RmtF , bla _OXA-232, ST15, aminoglycoside