Abstract: A linezolid-resistant E.faecalis  strain harboring optrA  and cfr  resistance genes were isolated from a patient in china, which had no mutations in rplC, rplD, rplV, and 23S rRNA gene. Transformation indicated that optrA  and cfr  were located on two different plasmids and both could be transferred to recipient strain, resulting in the increase of MICs of linezolid and chloramphenicol. Cfr , carried by an 11,872-bp plasmid, was enclosed with an IS 110  transposase in upstream and an IS 3 -like transposase in downstream, while optrA  was on an 8357-bp plasmid. As far as we know, this is the first report of an E.faecalis  clinical strain co-harboring optrA  and cfr  in China.
Keywords: E.faecalis , linezolid, cfr , optrA