Introduction: Aim to obtain a NO donor that can control released NO in vivo with the high efficacy of tumor suppression and targeting, a nanoplatform consisting of FA-Fe₃O₄@mSiO₂-Au/DOX was constructed.
Methods: In vitro, the nanoplatform catalyzed NO’s release with the maximum value of 4.91 μM within 60 min at 43°C pH=5.0, which was increased by 1.14 times when the temperature was 37°C. In vivo, 11.7 μg Au in the tumor tissue was found to catalyze S-nitrosoglutathione continuously, and 54 μM NO was checked out in the urine.
Results and Discussion: The high concentration of NO was found to increase the apoptotic rate and to reduce tumor proliferation. In the chemo-photothermal combination therapy, the tumor inhibition rate was increased up to 94.3%, and Au’s contribution from catalyzing NO release NO was 8.17%.
Keywords: thermotherapy, chemotherapy, NO control release, magnetic mesoporous silica nanocomposites