Abstract: Direct-on-target microdroplet growth assay is a new technique for analysing bacterial sensitivity and mechanisms of resistance. It is based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and allows for easy and rapid testing. Here, we describe the development and procedure of the direct-on-target microdroplet growth assay and summarise the latest clinical applications.
Keywords: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF MS, direct-on-target microdroplet growth assay, DOT-MGA, antimicrobial susceptibility testing