Purpose: Beta-lactamase-producing Klebsiella pneumoniae is common in the clinic, but research associated with the co-existence of KPC-2, LAP-2, and CTX-M-65 in K. pneumoniae is still rare. In this study, the phenotypic and genetic characteristics of a multidrug-resistant K. pneumoniae strain SJ25 co-harboring bla _KPC-2, bla _LAP-2, and bla _CTX-M-65 with rare ST1469 were investigated.
Methods and Results: Antimicrobial susceptibility testing revealed that strain SJ25 was resistant to various common antibiotics, except ciprofloxacin, fosfomycin, colistin, and tigecycline. Whole-genome analysis revealed that strain SJ25 carries a variety of antimicrobial resistance genes and virulence determinants. Plasmid analysis confirmed that the bla _KPC-2 and bla _CTX-M-65 genes were located on an ~136 kb transferrable IncFII/IncR plasmid and that bla _LAP-2 was located on an untypeable plasmid.
Conclusion: Our findings emphasized the need for continuous surveillance of β-lactamase-bearing K. pneumoniae in the clinic to control potential dissemination and outbreak.
Keywords: Klebsiella pneumoniae , multidrug-resistant, bla _KPC-2, bla _LAP-2, bla _CTX-M-65