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已发表论文
一项将传统中医与网络药理学相结合的多维度研究：探究五色植物特定组合的皮肤修复与抗衰老功效
Received 1 August 2025
Accepted for publication 16 November 2025
Published 28 November 2025 Volume 2025:18 Pages 3257—3269
DOI https://doi.org/10.2147/CCID.S552093
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Jeffrey Weinberg
Qinqian Tao, Xingmei Chen, Erping Yan
R&D Management Department, Guangzhou Natuspace Biotechnology Co., Ltd., Guangzhou, Guangdong, People’s Republic of China
Correspondence: Erping Yan, R&D Management Department, Guangzhou Natuspace Biotechnology Co., Ltd., Office Building B1, No. 2, Huanbao 3rd Road, Xintang Town, Zengcheng District, Guangzhou, Guangdong, People’s Republic of China, Email jsyan6944@iCloud.com
Background: Skin repair and anti-aging have long been central themes in dermatological and cosmetic science. Rooted in thousands of years of empirical knowledge, Traditional Chinese Medicine (TCM) literature contains systematic records of medicinal plants, many of which have been historically applied to improve skin health.
Objective: Based on the theoretical framework of the Five Elements, this study formulated a specific combination consisting of five chromatically representative medicinal plants—mung bean (green), white truffle (white), black truffle (black), chrysanthemum (yellow), and Lycium barbarum (red)—to investigate their potential synergistic anti-aging and skin regenerative effects.
Methods: The five-colored plants combination was analyzed by network pharmacology, followed by a scratch test of human fibroblasts, β-galactosidase staining, and ex vivo skin model.
Results: The network pharmacological analysis identified 27 core components and 636 targets, including CBS, CDK5, and AKR1B10, which are involved in skin anti-aging and repair. In vitro experiments indicated that at 0.5% concentration, the five-colored plant combination achieved a fibroblast wound healing rate of 59.89% after 24 hours, compared to the negative control group, which was 22.83% higher than that of individual raw materials. In the β-galactosidase staining of human fibroblasts, the blue-stained area in the experimental group was 0.777%, representing a 76.32% reduction compared to the negative control. In the in vitro skin model, treatment with the five-colored plant combination significantly increased the thickness of the epidermal living cell layer, collagen fiber content, and type IV collagen content, with respective increases of 28.16%, 49.12%, and 100.00%. Histological analysis revealed a significant increase in epidermal thickness and a more pronounced the dermal-epidermal junction (DEJ) ridge structure.
Conclusion: This study showed that the five-colored plant combination consisting of mung bean, white truffle, black truffle, chrysanthemum, and Lycium barbarum can repair the skin and delay aging.
Keywords: traditional Chinese medicine, five-colored plants combination, skin repair, anti-aging
R&D Management Department, Guangzhou Natuspace Biotechnology Co., Ltd., Guangzhou, Guangdong, People’s Republic of China
Correspondence: Erping Yan, R&D Management Department, Guangzhou Natuspace Biotechnology Co., Ltd., Office Building B1, No. 2, Huanbao 3rd Road, Xintang Town, Zengcheng District, Guangzhou, Guangdong, People’s Republic of China, Email jsyan6944@iCloud.com
Background: Skin repair and anti-aging have long been central themes in dermatological and cosmetic science. Rooted in thousands of years of empirical knowledge, Traditional Chinese Medicine (TCM) literature contains systematic records of medicinal plants, many of which have been historically applied to improve skin health.
Objective: Based on the theoretical framework of the Five Elements, this study formulated a specific combination consisting of five chromatically representative medicinal plants—mung bean (green), white truffle (white), black truffle (black), chrysanthemum (yellow), and Lycium barbarum (red)—to investigate their potential synergistic anti-aging and skin regenerative effects.
Methods: The five-colored plants combination was analyzed by network pharmacology, followed by a scratch test of human fibroblasts, β-galactosidase staining, and ex vivo skin model.
Results: The network pharmacological analysis identified 27 core components and 636 targets, including CBS, CDK5, and AKR1B10, which are involved in skin anti-aging and repair. In vitro experiments indicated that at 0.5% concentration, the five-colored plant combination achieved a fibroblast wound healing rate of 59.89% after 24 hours, compared to the negative control group, which was 22.83% higher than that of individual raw materials. In the β-galactosidase staining of human fibroblasts, the blue-stained area in the experimental group was 0.777%, representing a 76.32% reduction compared to the negative control. In the in vitro skin model, treatment with the five-colored plant combination significantly increased the thickness of the epidermal living cell layer, collagen fiber content, and type IV collagen content, with respective increases of 28.16%, 49.12%, and 100.00%. Histological analysis revealed a significant increase in epidermal thickness and a more pronounced the dermal-epidermal junction (DEJ) ridge structure.
Conclusion: This study showed that the five-colored plant combination consisting of mung bean, white truffle, black truffle, chrysanthemum, and Lycium barbarum can repair the skin and delay aging.
Keywords: traditional Chinese medicine, five-colored plants combination, skin repair, anti-aging