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已发表论文
天然单糖和双糖异构体区分的分析策略：技术与应用
Authors Yang R, Deng Z , Liu J, Shi Z, Jiang Y
Received 15 August 2025
Accepted for publication 20 November 2025
Published 12 December 2025 Volume 2025:19 Pages 11073—11092
DOI https://doi.org/10.2147/DDDT.S561012
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 6
Editor who approved publication: Professor Anastasios Lymperopoulos
Rong Yang,1,2,* Ziwei Deng,3,* Jing Liu,1,2 Zhihua Shi,3 Yueping Jiang1– 3
1Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, People’s Republic of China; 2National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, People’s Republic of China; 3Department of Clinical Pharmacy, Hunan University of Medicine General Hospital, Huaihua, Hunan, 418000, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Zhihua Shi; Yueping Jiang, Email hyshizh88@163.com; jiangyueping@126.com
Abstract: The precise structural differentiation of natural mono- and disaccharide isomers is fundamental for understanding carbohydrate bioactivity. However, this task remains challenging due to their minimal structural variances, high polarity, and lack of chromophores. This review traces the evolution of analytical technologies designed to overcome these hurdles. Early derivatization - GC-MS methods provided the foundation for isomer separation. Modern liquid chromatography-mass spectrometry (LC-MS), particularly with advanced chiral columns, now delivers high-resolution isomer profiling. Ion mobility spectrometry (IM-MS) further distinguishes conformers by their collision cross-section (CCS) values. Recent breakthroughs in non-derivatization strategies like UHPLC, coupled with the definitive structural validation offered by 2D-NMR, have revolutionized the field. We critically evaluate these methods’ detection limits and throughput for practical applications in food chemistry, clinical glycomics, and pharmaceutical analysis. Looking forward, emerging directions such as AI-assisted spectral interpretation and integrated microfluidic systems promise to propel glycan analysis toward rapid, precise, and intelligent diagnostics. This review provides a practical roadmap for selecting and advancing analytical techniques for real-world isomer characterization.
Keywords: monosaccharide, disaccharide, isomer, identification methods, derivatization, non-derivatization
1Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, People’s Republic of China; 2National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, People’s Republic of China; 3Department of Clinical Pharmacy, Hunan University of Medicine General Hospital, Huaihua, Hunan, 418000, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Zhihua Shi; Yueping Jiang, Email hyshizh88@163.com; jiangyueping@126.com
Abstract: The precise structural differentiation of natural mono- and disaccharide isomers is fundamental for understanding carbohydrate bioactivity. However, this task remains challenging due to their minimal structural variances, high polarity, and lack of chromophores. This review traces the evolution of analytical technologies designed to overcome these hurdles. Early derivatization - GC-MS methods provided the foundation for isomer separation. Modern liquid chromatography-mass spectrometry (LC-MS), particularly with advanced chiral columns, now delivers high-resolution isomer profiling. Ion mobility spectrometry (IM-MS) further distinguishes conformers by their collision cross-section (CCS) values. Recent breakthroughs in non-derivatization strategies like UHPLC, coupled with the definitive structural validation offered by 2D-NMR, have revolutionized the field. We critically evaluate these methods’ detection limits and throughput for practical applications in food chemistry, clinical glycomics, and pharmaceutical analysis. Looking forward, emerging directions such as AI-assisted spectral interpretation and integrated microfluidic systems promise to propel glycan analysis toward rapid, precise, and intelligent diagnostics. This review provides a practical roadmap for selecting and advancing analytical techniques for real-world isomer characterization.
Keywords: monosaccharide, disaccharide, isomer, identification methods, derivatization, non-derivatization