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已发表论文
用于治疗小鼠烟曲霉角膜炎的二硫化钼纳米片的抗真菌和抗炎疗法
Authors Feng Z , Lin J, Wang Q, Zhang L, Gu L, Yu B, Fu X , Li D, Zhao G, Li C
Received 27 July 2025
Accepted for publication 29 October 2025
Published 10 December 2025 Volume 2025:20 Pages 14837—14851
DOI https://doi.org/10.2147/IJN.S552664
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Kamakhya Misra
Zhuhui Feng,1 Jing Lin,1 Qian Wang,1 Lina Zhang,1 Lingwen Gu,1 Bing Yu,1 Xueyun Fu,1 Daohao Li,2 Guiqiu Zhao,1 Cui Li1
1Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, People’s Republic of China; 2State Key Laboratory of Bio-Fibers and Eco-Textiles, School of Environmental Science and Engineering, College of Materials Science and Engineering, Qingdao University, Qingdao, Shandong, People’s Republic of China
Correspondence: Guiqiu Zhao, Department of Ophthalmology, Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, Shandong, 266003, People’s Republic of China, Email zhaoguiqiu_good@126.com Cui Li, Department of Ophthalmology, Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, Shandong, 266003, People’s Republic of China, Email yankelicui@126.com
Purpose: To investigate the therapeutic potential of sulfur vacancy-molybdenum disulfide/carbon composite nanosheets (MoS2-x/C NS) in Aspergillus fumigatus (A. fumigatus) keratitis in mice.
Methods: The in vitro antifungal efficacy of MoS2-x/C NS against A. fumigatus was evaluated by propidium iodide (PI) staining, minimum inhibitory concentration (MIC) determination, and biofilm formation assays. Toxicity assessments of the MoS2-x/C NS were conducted using a Lactate dehydrogenase (LDH) assay kit for in vitro cytotoxicity and the Draize eye test for in vivo ocular irritation. The severity of fungal keratitis in mice was assessed using clinical scoring, plate counting, and hematoxylin and eosin (H&E) staining. The anti-inflammatory efficacy of MoS2-x/C NS was determined by quantifying inflammatory factor levels using reverse transcription polymerase chain reaction (RT-PCR).
Results: In vitro, MoS2−x/C NS significantly inhibited A. fumigatus growth, demonstrated favorable biocompatibility, and reduced the expression of IL-6 and TNF-α in human corneal epithelial cells (HCECs) stimulated by inactivated A. fumigatus hyphae. In vivo, MoS2−x/C NS treatment significantly reduced fungal load, attenuated pathological corneal damage, and suppressed IL-6 and TNF-α levels, effectively alleviating A. fumigatus keratitis in mice.
Conclusion: This study demonstrates that MoS2-x/C NS possesses significant therapeutic potential for fungal keratitis mediated through dual antifungal and anti-inflammatory mechanisms, thereby improving the prognosis of A. fumigatus keratitis.
Keywords: MoS2−x/C NS, sulfur vacancy, Aspergillus fumigatus keratitis, antifungal, anti-inflammatory, nanomaterial therapy
1Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, People’s Republic of China; 2State Key Laboratory of Bio-Fibers and Eco-Textiles, School of Environmental Science and Engineering, College of Materials Science and Engineering, Qingdao University, Qingdao, Shandong, People’s Republic of China
Correspondence: Guiqiu Zhao, Department of Ophthalmology, Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, Shandong, 266003, People’s Republic of China, Email zhaoguiqiu_good@126.com Cui Li, Department of Ophthalmology, Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, Shandong, 266003, People’s Republic of China, Email yankelicui@126.com
Purpose: To investigate the therapeutic potential of sulfur vacancy-molybdenum disulfide/carbon composite nanosheets (MoS2-x/C NS) in Aspergillus fumigatus (A. fumigatus) keratitis in mice.
Methods: The in vitro antifungal efficacy of MoS2-x/C NS against A. fumigatus was evaluated by propidium iodide (PI) staining, minimum inhibitory concentration (MIC) determination, and biofilm formation assays. Toxicity assessments of the MoS2-x/C NS were conducted using a Lactate dehydrogenase (LDH) assay kit for in vitro cytotoxicity and the Draize eye test for in vivo ocular irritation. The severity of fungal keratitis in mice was assessed using clinical scoring, plate counting, and hematoxylin and eosin (H&E) staining. The anti-inflammatory efficacy of MoS2-x/C NS was determined by quantifying inflammatory factor levels using reverse transcription polymerase chain reaction (RT-PCR).
Results: In vitro, MoS2−x/C NS significantly inhibited A. fumigatus growth, demonstrated favorable biocompatibility, and reduced the expression of IL-6 and TNF-α in human corneal epithelial cells (HCECs) stimulated by inactivated A. fumigatus hyphae. In vivo, MoS2−x/C NS treatment significantly reduced fungal load, attenuated pathological corneal damage, and suppressed IL-6 and TNF-α levels, effectively alleviating A. fumigatus keratitis in mice.
Conclusion: This study demonstrates that MoS2-x/C NS possesses significant therapeutic potential for fungal keratitis mediated through dual antifungal and anti-inflammatory mechanisms, thereby improving the prognosis of A. fumigatus keratitis.
Keywords: MoS2−x/C NS, sulfur vacancy, Aspergillus fumigatus keratitis, antifungal, anti-inflammatory, nanomaterial therapy