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已发表论文
靶向抑瘤素 M 基因的抗炎组合用于重塑大鼠肛周溃疡炎症肠道菌群的研究
Authors Wu Y, Ge H, Zhao H, Zou K, Wang P, Wang Y, Zhang Y
Received 10 June 2025
Accepted for publication 19 November 2025
Published 17 December 2025 Volume 2025:18 Pages 17769—17788
DOI https://doi.org/10.2147/JIR.S546010
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Dr Anish R. Maskey
Yanlan Wu,1,* Hao Ge,2,* Haoran Zhao,3 Kaiping Zou,4 Pei Wang,5 Yi Wang,1 Yang Zhang5
1Department of Colon and Rectal Surgery, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China; 2School of Clinical Medicine, Jiangsu Health Vocational College, Nanjing, Jiangsu, People’s Republic of China; 3First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China; 4Department of Pharmacy, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China; 5Jiangsu Clinical Innovation Center for Anorectal Diseases of Traditional Chinese Medicine (TCM), Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Yang Zhang, Jiangsu Clinical Innovation Center for Anorectal Diseases of Traditional Chinese Medicine (TCM), Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China, Email fsyy00239@njucm.edu.cn
Background: Perianal Abscess is an inflammatory disease caused by infection in the perianal area, characterized by inflammatory cell infiltration and imbalance of intestinal flora. The herbal medicine anti-inflammatory combination (KYHJ) has therapeutic effects in acute and chronic soft tissue infections, but the specific therapeutic mechanism in perianal inflammatory diseases is unclear. There is a literature evidence showing the role of OSM gene in inflammatory conditions, however this was not previously studied in perianal abscess.
Methods: A perianal inflammation model was constructed in SD rats using 75% glacial acetic acid and treated with different doses of KYHJ; genome-wide changes were detected by RNA sequencing. H&E staining observed pathological states, TUNEL kit detected apoptosis, WB measured apoptotic protein levels, ELISA detected inflammatory factors in serum, and 16S rRNA sequencing analyzed intestinal flora abundance. In vitro, an anal epithelial cell inflammation model induced by LPS was treated with 10% KYHJ-containing serum; EDU assay, flow cytometry, WB, and ELISA were used to detect cell proliferation, apoptosis, related protein levels, and inflammatory factor secretion. Oncostatin M gene was knocked down in rats and overexpressed in epithelial cells for mechanism exploration.
Results: Results showed that KYHJ ameliorated perianal tissue inflammatory infection, inhibited apoptosis, and restored intestinal flora abundance. Initial transcriptome analysis, RT-qPCR and WB performed in this study have additionally supported the role of OSM gene. RNA sequencing linked the tested anti-inflammatory effects of KYHJ to reduced Oncostatin M (OSM) gene expression. RNA transcriptome sequencing showed high Oncostatin M expression in inflamed rats and low expression in the KYHJ group; in vivo knockdown improved perianal inflammation and increased flora abundance. In vitro, KYHJ - containing serum inhibited LPS - induced apoptosis, promoted proliferation, and reduced inflammatory factor secretion, which were reversed by Oncostatin M overexpression.
Conclusion: KYHJ ameliorates perianal inflammatory diseases by targeting Oncostatin M, restoring intestinal flora imbalance, promoting cell proliferation, and inhibiting apoptosis.
Plain Language Summary: We investigated the effects of KYHJ in a rat model of perianal inflammation and in cell experiments. In the rat model, the Oncostatin M (OSM) gene was highly expressed during perianal inflammation but showed reduced expression after KYHJ treatment. KYHJ also modulated the gut microbiota, suppressed secretion of inflammatory factors, and alleviated clinical signs of perianal abscess in treated rats, likely via regulation of OSM. In vitro, serum containing KYHJ promoted proliferation and reduced apoptosis of LPS-treated anal epithelial cells, while decreasing their secretion of inflammatory mediators.
Keywords: anti-inflammatory combinations, oncostatin M, intestinal flora, perianal abscess, apoptosis
1Department of Colon and Rectal Surgery, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China; 2School of Clinical Medicine, Jiangsu Health Vocational College, Nanjing, Jiangsu, People’s Republic of China; 3First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China; 4Department of Pharmacy, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China; 5Jiangsu Clinical Innovation Center for Anorectal Diseases of Traditional Chinese Medicine (TCM), Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Yang Zhang, Jiangsu Clinical Innovation Center for Anorectal Diseases of Traditional Chinese Medicine (TCM), Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China, Email fsyy00239@njucm.edu.cn
Background: Perianal Abscess is an inflammatory disease caused by infection in the perianal area, characterized by inflammatory cell infiltration and imbalance of intestinal flora. The herbal medicine anti-inflammatory combination (KYHJ) has therapeutic effects in acute and chronic soft tissue infections, but the specific therapeutic mechanism in perianal inflammatory diseases is unclear. There is a literature evidence showing the role of OSM gene in inflammatory conditions, however this was not previously studied in perianal abscess.
Methods: A perianal inflammation model was constructed in SD rats using 75% glacial acetic acid and treated with different doses of KYHJ; genome-wide changes were detected by RNA sequencing. H&E staining observed pathological states, TUNEL kit detected apoptosis, WB measured apoptotic protein levels, ELISA detected inflammatory factors in serum, and 16S rRNA sequencing analyzed intestinal flora abundance. In vitro, an anal epithelial cell inflammation model induced by LPS was treated with 10% KYHJ-containing serum; EDU assay, flow cytometry, WB, and ELISA were used to detect cell proliferation, apoptosis, related protein levels, and inflammatory factor secretion. Oncostatin M gene was knocked down in rats and overexpressed in epithelial cells for mechanism exploration.
Results: Results showed that KYHJ ameliorated perianal tissue inflammatory infection, inhibited apoptosis, and restored intestinal flora abundance. Initial transcriptome analysis, RT-qPCR and WB performed in this study have additionally supported the role of OSM gene. RNA sequencing linked the tested anti-inflammatory effects of KYHJ to reduced Oncostatin M (OSM) gene expression. RNA transcriptome sequencing showed high Oncostatin M expression in inflamed rats and low expression in the KYHJ group; in vivo knockdown improved perianal inflammation and increased flora abundance. In vitro, KYHJ - containing serum inhibited LPS - induced apoptosis, promoted proliferation, and reduced inflammatory factor secretion, which were reversed by Oncostatin M overexpression.
Conclusion: KYHJ ameliorates perianal inflammatory diseases by targeting Oncostatin M, restoring intestinal flora imbalance, promoting cell proliferation, and inhibiting apoptosis.
Plain Language Summary: We investigated the effects of KYHJ in a rat model of perianal inflammation and in cell experiments. In the rat model, the Oncostatin M (OSM) gene was highly expressed during perianal inflammation but showed reduced expression after KYHJ treatment. KYHJ also modulated the gut microbiota, suppressed secretion of inflammatory factors, and alleviated clinical signs of perianal abscess in treated rats, likely via regulation of OSM. In vitro, serum containing KYHJ promoted proliferation and reduced apoptosis of LPS-treated anal epithelial cells, while decreasing their secretion of inflammatory mediators.
Keywords: anti-inflammatory combinations, oncostatin M, intestinal flora, perianal abscess, apoptosis