Jia Meng,1 Yuying Wang,1 Qinglong Cao,1 Mengfan Li,1 Qibin Zhang,1 Mingchao Zhang,2 Yadi Wang,3 Yang Liu,1 Lin Tang4 

1Department of Public Health, Jinzhou Medical University, Jinzhou, Liaoning, People’s Republic of China; 2Department of Orthopedics, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, People’s Republic of China; 3Department of Precision Medicine, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, People’s Republic of China; 4Biomarker Technologies Corporation, Beijing, People’s Republic of China

Correspondence: Jia Meng, Department of Public Health, Jinzhou Medical University, No. 40, Section 3, Songpo Road, Linghe District, Jinzhou, Liaoning, 121001, People’s Republic of China, Email mengj@jzmu.edu.cn
摘要：

目的：下丘脑视交叉上核（SCN）是昼夜节律的主生物钟，协调人体整个昼夜节律系统，包括褪黑素的分泌。方法：本研究旨在阐明蓝光对SD大鼠转录组谱的影响。实验组大鼠在夜间分别暴露于450nm和465nm波长的蓝光下3小时和6小时。经过6周蓝光干预后，取视网膜组织进行组织学分析，采集血液样本进行ELISA检测，收集下丘脑样本进行全长RNA测序。通过RT-qPCR和Western blot验证差异表达基因。通过相关性分析评估节律基因表达水平与血中褪黑素及AA-NAT浓度之间的关系。结果：研究结果表明，暴露于蓝光的SD大鼠视网膜细胞结构受到损伤，其血中褪黑素和AA-NAT水平出现紊乱。鉴定出差异表达基因，KEGG分析显示TGF-β信号通路可能与蓝光暴露相关。尽管样本量和差异参数存在局限性，但通过RT-qPCR分析验证了结果的准确性和可靠性，证实结果确实准确可靠。结论：本研究结果为阐明蓝光暴露的影响机制提供了新的见解，可能为通过调节昼夜节律来治疗光致病提供新的思路。