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已发表论文
转录组学和网络药理学揭示清玄润目饮治疗干眼症的抗炎和抗氧化应激机制
Authors Zhao S , Wang J, Liu Y , Hu X, Gu C, Yao J
Received 30 January 2025
Accepted for publication 26 June 2025
Published 8 January 2026 Volume 2026:19 516780
DOI https://doi.org/10.2147/JIR.S516780
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Professor Ning Quan
Shanshan Zhao,1,* Jiadi Wang,1,2,* Ying Liu,1 Xi Hu,1 Chenhao Gu,1 Jing Yao1,2
1Graduate School, Heilongjiang University of Chinese Medicine, Harbin, 150000, People’s Republic of China; 2Ophthalmology Department, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, 150000, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Jing Yao, Ophthalmology Department, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, 26 Heping Road, Xiangfang District, Harbin, Heilongjiang, 150000, People’s Republic of China, Tel +451-82111401, Email YJ00113@outlook.com
Purpose: Dry eye disease (DED) is a multifactorial chronic disorder of the ocular surface, characterized by reduced tear secretion, tear film instability, and inflammation. Oxidative stress is pivotal in DED pathogenesis. Qingxuan Runmu Yin (QRY) has shown substantial clinical efficacy in managing DED. This study aims to elucidate the molecular mechanisms underlying the antioxidant and anti-inflammatory effects of QRY in DED.
Methods: A benzalkonium chloride (BAC)-induced DED mouse model was established to evaluate QRY’s protective effects on the ocular surface. RNA sequencing identified differentially expressed genes (DEGs, fold change > 1.5, P < 0.05) among control, untreated DED, and QRY-treated DED mice. Transcriptomic analysis of DEGs was conducted. The active ingredients and targets of the ten Chinese herbal medicines in QRY were obtained from the TCMSP and TCMIP databases, whereas DED and oxidative stress-related genes were identified from the DisGeNET, OMIM, and GeneCards databases. A Chinese herbal medicine-active ingredient-target-disease network was constructed. The key pathways and targets of QRY against DED were investigated through transcriptomics and network pharmacology, and experimental validation was performed.
Results: QRY alleviated ocular surface damage in DED mice by repairing goblet cells, improving corneal damage, enhancing tear secretion, and reducing inflammatory factors and reactive oxygen species (ROS). Combined transcriptomic and network pharmacology analyses indicated that QRY reduced oxidative stress in DED by inhibiting pathways such as NF-κB and TNF. In vitro, QRY improved the survival of human corneal epithelial (HCE) cells damaged by oxidative stress. It inhibited the NF-κB signaling pathway and reduced levels of the pro inflammatory cytokines IL 1β, IL 6, and TNF α. In addition, QRY lowered ROS levels and enhanced superoxide dismutase (SOD) activity.
Conclusion: This study is the first to demonstrate that QRY mitigates ocular surface inflammation and oxidative stress in DED, providing a scientific foundation for its clinical application.
Keywords: dry eye disease, oxidative stress, inflammation, transcriptomics, network pharmacology, qingxuan runmu yin (QRY)
1Graduate School, Heilongjiang University of Chinese Medicine, Harbin, 150000, People’s Republic of China; 2Ophthalmology Department, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, 150000, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Jing Yao, Ophthalmology Department, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, 26 Heping Road, Xiangfang District, Harbin, Heilongjiang, 150000, People’s Republic of China, Tel +451-82111401, Email YJ00113@outlook.com
Purpose: Dry eye disease (DED) is a multifactorial chronic disorder of the ocular surface, characterized by reduced tear secretion, tear film instability, and inflammation. Oxidative stress is pivotal in DED pathogenesis. Qingxuan Runmu Yin (QRY) has shown substantial clinical efficacy in managing DED. This study aims to elucidate the molecular mechanisms underlying the antioxidant and anti-inflammatory effects of QRY in DED.
Methods: A benzalkonium chloride (BAC)-induced DED mouse model was established to evaluate QRY’s protective effects on the ocular surface. RNA sequencing identified differentially expressed genes (DEGs, fold change > 1.5, P < 0.05) among control, untreated DED, and QRY-treated DED mice. Transcriptomic analysis of DEGs was conducted. The active ingredients and targets of the ten Chinese herbal medicines in QRY were obtained from the TCMSP and TCMIP databases, whereas DED and oxidative stress-related genes were identified from the DisGeNET, OMIM, and GeneCards databases. A Chinese herbal medicine-active ingredient-target-disease network was constructed. The key pathways and targets of QRY against DED were investigated through transcriptomics and network pharmacology, and experimental validation was performed.
Results: QRY alleviated ocular surface damage in DED mice by repairing goblet cells, improving corneal damage, enhancing tear secretion, and reducing inflammatory factors and reactive oxygen species (ROS). Combined transcriptomic and network pharmacology analyses indicated that QRY reduced oxidative stress in DED by inhibiting pathways such as NF-κB and TNF. In vitro, QRY improved the survival of human corneal epithelial (HCE) cells damaged by oxidative stress. It inhibited the NF-κB signaling pathway and reduced levels of the pro inflammatory cytokines IL 1β, IL 6, and TNF α. In addition, QRY lowered ROS levels and enhanced superoxide dismutase (SOD) activity.
Conclusion: This study is the first to demonstrate that QRY mitigates ocular surface inflammation and oxidative stress in DED, providing a scientific foundation for its clinical application.
Keywords: dry eye disease, oxidative stress, inflammation, transcriptomics, network pharmacology, qingxuan runmu yin (QRY)