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比较蛋白质组学分析揭示糙叶败酱对耐甲氧西林表皮葡萄球菌的抗菌机制
Authors Liu X, An L, Ren S, Zhou Y, Peng W
Received 22 November 2021
Accepted for publication 21 January 2022
Published 6 March 2022 Volume 2022:15 Pages 883—893
DOI https://doi.org/10.2147/IDR.S350715
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Professor Héctor M Mora-Montes
Purpose: As a kind of opportunist pathogen, Staphylococcus epidermidis (MRSE) can cause nosocomial infections and easily evolve into resistant bacteria. Among these, methicillin-resistant Staphylococcus epidermidis (MRSE) exhibit significantly higher rates. Our previous study showed that Patrinia scabiosaefolia (PS) possessed strong antibacterial activity against MRSE. However, the mechanism of PS against MRSE is not clear.
Methods: Here, a tandem mass tag-based (TMT) proteomic analysis was performed to elucidate the potential mechanism of PS against MRSE. We compared the differential expression proteins of MRSE under PS stress.
Results: Based on a fold change of > 1.2 or < 1/1.2 (with p value set at < 0.05), a total of 248 proteins (128 up-regulated proteins, 120 down-regulated proteins) were identified. Bioinformatic analysis showed that proteins including arginine deiminase (arcA ), ornithine carbamoyltransferase (arcB ) and carbamate kinase (arcC ), serine–tRNA ligase (serS ), phenylalanine–tRNA ligase beta and subunit (pheT ), DltD (dlt ), d-alanyl carrier protein (dlt ), accumulation-associated protein (SasG ), serine-aspartate repeat-containing protein C (SdrC ) and hemin transport system permease protein HrtB (VraG ) played important roles in mechanism of PS against MRSE.
Conclusion: In summary, these results indicated that arginine deiminase pathway (ADI) pathway, protein synthesis, cell wall synthesis, biofilm formation and uptake of iron were related to mechanisms of PS against MRSE. Our findings provide an insight into the the mechanism of PS against MRSE, and may be valuable in offering new targets to develop more anti-MRSE drugs.
Keywords: methicillin-resistant Staphylococcus epidermidis , Patrinia scabiosaefolia , proteomic