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芹菜素-7-O-葡糖苷对金黄色葡萄球菌和大肠杆菌的抗生物膜活性及机制
Authors Pei ZJ, Li C, Dai W, Lou Z, Sun X, Wang H, Khan AA, Wan C
Received 22 August 2022
Accepted for publication 22 January 2023
Published 11 April 2023 Volume 2023:16 Pages 2129—2140
DOI https://doi.org/10.2147/IDR.S387157
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Professor Suresh Antony
Introduction: This study aimed to examine the anti-biofilm activity and mechanism of gallic acid (GA), kaempferol-7-O-glucoside (K7G) and apigenin-7-O-glucoside (A7G) against Staphylococcus aureus and Escherichia coli.
Methods: The antibacterial activity of the natural compounds was determined by serial dilution method. The inhibitory activity of natural compounds on biofilms was determined by crystal violet staining method. The effects and mechanisms of natural compounds on bacterial biofilms were analyzed by atomic force microscopy.
Results: In our study, compared with GA and K7G, A7G was found to exhibit the strongest anti-biofilm and antibacterial activities. The minimum biofilm inhibitory concentration (MBIC) of A7G against S. aureus and E. coli was 0.20 mg/mL and 0.10 mg/mL, respectively. The inhibition rates of 1/2 MIC of A7G on biofilms of S. aureus and E. coli were 88.9%, and 83.2% respectively. Moreover, atomic force microscope (AFM) images showed the three-dimensional biofilm morphology of S. aureus and E. coli , and the results indicated that A7G was highly effective in biofilm inhibition.
Discussion: It was found that the inhibition of A7G on biofilm was achieved through inhibiting on exopolysaccharides (EPS), quorum sensing (QS), and cell surface hydrophobicity (CSH). A7G exerted strong anti-biofilm activities by inhibiting EPS production, QS, and CSH. Hence, A7G, as a natural substance, could be a promising novel antibacterial and anti-biofilm agent for control of biofilm in food industry.
Keywords: apigenin-7-O-glucoside, anti-biofilm, exopolysaccharide, quorum sensing, cell surface hydrophobicity, atomic force microscope