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Authors Liu XX, Liao YH, Wang XX, Zou DH, Luo C, Jian CD, Wu Y
Received 4 May 2017
Accepted for publication 20 June 2017
Published 31 July 2017 Volume 2017:13 Pages 2037—2044
DOI https://doi.org/10.2147/NDT.S141062
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Prof. Dr. Roumen Kirov
Peer reviewer comments 2
Editor who approved publication: Professor Wai Kwong Tang
Abstract: MicroRNA
(miRNA) is believed to play a crucial role in the cause and treatment of
epilepsy by controlling gene expression. However, it is still unclear how miRNA
profiles change after multiple prolonged seizures and aggravation of brain
injury in chronic epilepsy (CE). To investigate the role of miRNA in epilepsy,
we utilized the CE rat models with pentylenetetrazol (PTZ) and miRNA profiles
in the hippocampus. miRNA profiles were characterized using miRNA microarray
analysis and were compared with the rats in the sham group, which received 0.9%
physiological saline treatment at the same dose. Four up-regulated miRNAs
(miR-139–3p, -770–5p, -127–5p, -331–3p) and 5 down-regulated miRNAs
(miR-802–5p, -380–5p, -183–5p, -547–5p, -344a/-344a–5p) were found in the CE
rats (fold change >1.5, P <0.05). Three
of the dysregulated miRNAs were validated by quantitative real-time polymerase
chain reaction, which revealed an outcome consistent with the initial results
of the miRNA microarray analyses. Then, miR-344a agomir was
intracerebroventricularly injected and followed by PTZ induction of CE models
to investigate the effect of miR-344a in chronic neocortical epileptogenesis.
After miRNA-344a agomir and scramble treatment, results showed a restoration of
seizure behavior and a reduction in neuron damage in the cortex in miRNA-334a
agomir treated rats. These data suggest that miRNA-344a might have a small
modulatory effect on seizure-induced apoptosis signaling pathways in the
cortex.
Keywords: microRNA, chronic epilepsy, miR-344a, epigenetics, apoptosis