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Authors Diao L, Wang S, Sun Z
Received 9 February 2018
Accepted for publication 21 March 2018
Published 14 May 2018 Volume 2018:11 Pages 2797—2804
DOI https://doi.org/10.2147/OTT.S165147
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Colin Mak
Peer reviewer comments 2
Editor who approved publication: Dr XuYu Yang
Background: Dysregulated long noncoding RNAs (lncRNAs) and microRNAs (miRNAs)
play key roles in the development of human cancers. LncRNA GAPLINC has been
reported to be increased in gastric cancer (GC) tissues.
Methods: Real-time PCR assays were used to measure expressions of GAPLINC,
miR-378, and MAPK1 mRNA. Western blot assays were employed to examine MAPK1
protein expression. Cell proliferation and cell cycle were measured by CCK-8
and propidium iodide-detection assays, respectively. The interaction between
GAPLINC and miR-378 was confirmed by site-directed mutagenesis and luciferase
assays. Luciferase assays were also used to study whether GAPLINC was able to
act as a molecular sponge of miR-378 to modulate MAPK1 expression.
Results: The lncRNA GAPLINC expression was upregulated and positively correlated
with MAPK1 expression in gastric cancer tissues and cells. Additionally, lncRNA
GAPLINC promoted the expression of MAPK1 and the enhancement of GC cell
proliferation and cell cycle progression by LncRNA GAPLINC was dependent on
MAPK1 in vitro and in vivo. Consequently, we found that miR-378 expression was
inversely correlated with GAPLINC expression in GC tissues and cells. miR-378
could directly bind to GAPLINC and decreased GAPLINC expression, thus reducing
MAPK1 expression. Furthermore, overexpression of miR-378 inhibited MAPK1
expression, cell proliferation, and cell cycle progression of gastric cancer
cells, while these effects were abrogated by upregulating lncRNA GAPLINC
expression.
Conclusion: Taken together, lncRNA GAPLINC promotes gastric cancer cell
proliferation by acting as a molecular sponge of miR-378 to modulate MAPK1
expression.
Keywords: GAPLINC, cell proliferation, miR-378, MAPK1, gastric cancer