论文已发表
注册即可获取德孚的最新动态
IF 收录期刊
Authors Yang X, Qiu J, Kang H, Wang Y, Qian J
Received 9 January 2018
Accepted for publication 3 May 2018
Published 16 November 2018 Volume 2018:10 Pages 5839—5853
DOI https://doi.org/10.2147/CMAR.S161990
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Andrew Yee
Peer reviewer comments 5
Editor who approved publication: Dr Leylah Drusbosky
Background: MiR-125a-5p has
been reported to be involved in the development and progression of various
cancers. However, the biological function and underlying mechanisms in
colorectal cancer(CRC) still remain unclear. Here, we explored the potential
biological roles of miR-125a-5p in CRC.
Methods: The
expression of miR-125a-5p was detected using quantitative real-time PCR
(qRT-PCR), biological functions of miR-125a-5p were assessed by cell counting
kit-8, wound-healing, transwell invasion, and human umbilical vein endothelial
cell (HUVEC) tube formation assays in vitro and animal experiments in
vivo.
Results: We found
that miR-125a-5p was downregulated in CRC tissues and cell lines, it inhibited
CRC cell proliferation, migration, and invasion and reduced the ability of
HUVECs to form tubes. Moreover, we verifed that miR-125a-5p suppressed CRC
growth and metastasis in vivo. Additionally, we showed that VEGFA, a direct
target gene of miR-125a-5p, could reverse the inhibitory effect caused by
miR-125a-5p overexpression.
Conclusion: miR-125a-5p
might serve as a tumor suppressor in CRC and could be regarded as a potential
therapeutic candidate for CRC.
Keywords: miR-125a-5p,
colorectal cancer, VEGFA, overall survival