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Authors Sun H, Xi P, Sun Z, Wang Q, Zhu B, Zhou J, Jin H, Zheng W, Tang W, Cao H, Cao X
Received 28 April 2018
Accepted for publication 1 September 2018
Published 16 November 2018 Volume 2018:10 Pages 5725—5734
DOI https://doi.org/10.2147/CMAR.S172592
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Andrew Yee
Peer reviewer comments 2
Editor who approved publication: Professor Nakshatri
Background: Circular
RNAs(circRNAs) have been reported as a diverse class of endogenous RNA that
regulate gene expression in eukaryotes. Recent evidence suggested that many
circular RNAs can act as oncogenes or tumor suppressors through sponging
microRNAs. However, the function of circular RNAs in gastric cancer remains
largely unknown.
Materials and methods: The circRNA
levels in gastric carcinoma tissues and plasmas were detected by real-time
quantitative reverse transcription-polymerase chain reaction. The correlation
between the expression of circRNA and clinic pathological features was
analyzed. Rate of inhibiting of proliferation was measured using a CCK-8 cell
proliferation assay. Clone formation ability was assessed with a clone
formation inhibition test. We used the bioinformatics software to predict
circRNA-miRNA and miRNA-mRNA interactions. Relative gene expression was
assessed using quantitative real-time polymerase chain reaction and relative
protein expression levels were determined with western blotting. CircRNA and
miRNA interaction was confrmed by dual-luciferase reporter assays.
Results: We
characterized that one circRNA named circ-SFMBT2 showed an increased expression
level in gastric cancer tissues compared to adjacent non-cancerous tissues and
was associated with higher tumor stages of gastric cancer. Silencing of
circ-SFMBT2 inhibited the proliferation of gastric cancer cells significantly.
Importantly, we demonstrated that circ-SFMBT2 could act as a sponge of
miR-182-5p to regulate the expression of CREB1 mRNA, named as cAMP response
element binding protein 1, and further promote the proliferation of gastric
cancer cells.
Conclusion: Our study
reveals that circ-SFMBT2 participates in progression of gastric cancer by
competitively sharing miR-182-5p with CREB1, providing a novel target to
improve the treatment of gastric cancer.
mutation-analysis-of-beta-thalassemia-in-east-western-indian-populatio-peer-reviewed-article-TACG
for an example.
Keywords: tissues,
plasmas, target, progression, AGO2, sponge