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Authors Jiang J, Xia Y, Liang Y, Yang M, Zeng W, Zeng X
Received 7 August 2018
Accepted for publication 11 October 2018
Published 20 November 2018 Volume 2018:13 Pages 3777—3790
DOI https://doi.org/10.2147/COPD.S182504
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Amy Norman
Peer reviewer comments 2
Editor who approved publication: Professor Chunxue Bai
Purpose: miR-190a-5p
expression alters dynamically in response to hypoxia. However, the role of
miR-190a-5p expression in hypoxia-induced pulmonary hypertension (PH) remains
unclear. We sought to correlate the miR-190a-5p expression levels with the
severity, diagnosis, and prognosis of PH in relation to chronic obstructive
pulmonary disease (COPD-PH). Additionally, we evaluated the effect of
miR-190a-5p through in vitro experiments on human pulmonary endothelial cells
(HPECs) that were exposed to hypoxia and in vivo experiments using an animal
model of hypoxia-induced PH.
Methods: Circulating
miR-190a-5p levels were measured from 73 patients with PH and 32 healthy
controls through quantitative real-time PCR. The levels of miR-190a-5p and the
expression of Krüppel-like factor 15 (KLF15) were analyzed in HPECs that were
exposed to hypoxia, and the effects of antagomir-190a-5p in mice with chronic
hypoxia-induced PH were tested. Target gene analysis was performed by Western
blot and luciferase assay.
Results: The
miR-190a-5p level was significantly higher in patients with COPD-PH than in the
healthy controls. Higher miR-190a-5p levels were associated with a greater
severity of COPD-PH. In vitro experiments on HPECs showed that exposure to
hypoxia increased the miR-190a-5p levels significantly. KLF15 was validated as
a target of miR-190a-5p. Transfection with miR-190a-5p mimicked inhibition of
KLF15 expression in HPECs. In the mouse model of PH, antagomir-190a-5p reduced
right ventricular systolic pressure and enhanced the KLF15 expression levels in
lung tissue.
Conclusion: miR-190a-5p
regulates hypoxia-induced PH by targeting KLF15. The circulating levels of
miR-190a-5p correlate with the severity of COPD-PH, thereby confirming the
diagnostic and prognostic value of this parameter in COPD-PH.
Keywords: hypoxia,
pulmonary hypertension, COPD, miR-190a-5p, KLF15