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Authors Li N, Cui M, Yu P, Li Q
Received 22 October 2018
Accepted for publication 17 January 2019
Published 18 February 2019 Volume 2019:12 Pages 1269—1278
DOI https://doi.org/10.2147/OTT.S191700
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Amy Norman
Peer reviewer comments 2
Editor who approved publication: Dr Arseniy Yuzhalin
Background: Cervical
lymph node metastasis is an important prognostic indicator for papillary
thyroid carcinoma (PTC) and affects treatment strategies for PTC. lncRNAs
essentially contribute to the biological functions of tumors. This study aimed
to identify the lncRNAs associated with cervical lymph node metastasis and
prognosis of PTC and their potential pathophysiological mechanisms.
Materials and methods: PTC-associated
lncRNAs were selected from The Cancer Genome Atlas database, and correlations
among lncRNAs, lymph node metastasis, tumor staging, and prognosis of PTC were
analyzed in silico. These correlations were then validated through quantitative
reverse transcription PCR (qRT-PCR) and immunohistochemistry (IHC).
Results: In silico
analysis showed that FAM95B1 and UCA1 were
significantly correlated with cervical lymph node metastasis, tumor staging,
and PTC prognosis (P <0.05). qRT-PCR analysis revealed high UCA1
expression in PTC tissues and correlations between UCA1 expression levels and
cervical lymph node metastasis and tumor staging in PTC, that is, higher UCA1 expression
resulted in poorer PTC prognosis. IHC analysis revealed that a high expression
of UCA1 was
accompanied by a high expression of metastasis-related proteins (MMP-2 and
MMP-9), thereby validating the correlation of UCA1 expression
with metastasis.
Conclusion: FAM95B1 and UCA1 expression
was significantly correlated with the occurrence and progression of PTC. The
expression levels of UCA1 significantly affected the prognosis of PTC
patients and were significantly correlated with tumor staging and cervical
lymph node metastasis.
Keywords: papillary
thyroid carcinoma, lncRNA, FAM95B1 , UCA1