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Authors Yu SY, Zhang L, Chen S, Kong F, Xiao M, Wang H, Hou X, Zhou ML, Zhang G, Zhang JJ, Duan SM, Kang W, Xu YC
Received 24 December 2018
Accepted for publication 19 March 2019
Published 18 April 2019 Volume 2019:12 Pages 865—875
DOI https://doi.org/10.2147/IDR.S199359
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Cristina Weinberg
Peer reviewer comments 2
Editor who approved publication: Dr Joachim Wink
Introduction: We studied
the species distribution and antifungal susceptibilities of Candida isolates
causing refractory or recurrent oropharyngeal candidiasis (OPC) in a
multicenter study in China (2013–2016).
Methods: Species
identification was performed using the Bruker Biotyper (Bruker Daltonics,
Germany) matrix-assisted laser desorption/ionization time of flight mass
spectrometry system supplemented by internal transcribed spacer sequencing as
required. Antifungal susceptibilities were determined by the Clinical and Laboratory
Standards Institute document (CLSI) M27-A3 broth microdilution methodology.
Results: A total
of 558 non-duplicate Candida isolates comprising 10 species were
obtained from 535 patients. Candida albicans was the most common species
(89.6%), followed by C. glabrata (5.2%), C. tropicalis (2.9%),
and C.
parapsilosis (0.7%). Azoles were active against C. albicans with
susceptibility rates of 96% and 95.8% for fluconazole and voriconazole,
respectively. MIC50 values of C. albicans to
fluconazole, voriconazole, itraconazole, and miconazole were 1, 0.03, 0.25 and
0.12 μg/mL, respectively, higher than those in previous studies of which OPC
patients (corresponding MIC50 values of 0.25 , 0.015 , 0.06 , and 0.03
μg/mL). Except for itraconazole, the MIC50 and MIC90 values of 58
non-C.
albicans to other azoles were two to threefold higher
than C.
albicans . Miconazole, amphotericin B, nystatin, and 5-flucytosine
had good in vitro antifungal activity for all isolates.
Conclusion: The study
provides valuable data on the species distribution and antifungal
susceptibility of oropharyngeal Candida isolates from geographically diverse
areas of China. C. albicans remains the most common species but
with increasing rates of azoles resistance.
Keywords: oral
candidiasis, Candida, identification, antifungal susceptibility