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Authors Liu Z, Xu Z, Tian Y, Yan H, Lou Y
Received 28 October 2018
Accepted for publication 11 February 2019
Published 18 April 2019 Volume 2019:12 Pages 3031—3042
DOI https://doi.org/10.2147/OTT.S192553
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Cristina Weinberg
Peer reviewer comments 2
Editor who approved publication: Dr Federico Perche
Background: ZNF277 is
a transcription factor that is overexpressed in several cancers. However, its
clinical role in ovarian cancer (OC) has not been reported yet. The present
study aims to investigate the expression of ZNF277 in patients with OC, and to
reveal the effects of ZNF277 on the proliferation, migration, and invasion of
OC cells.
Methods: Using The
Cancer Genome Atlas database, we found that higher expression of ZNF277 was
correlated with poorer survival times of OC patients. This study used
functional experiments, such as Cell Counting Kit-8 assay, colony formation
assay, wound healing assay, and transwell invasion assay. Mechanistically,
using quantitative chromatin immunoprecipitation assay, luciferase reporter
assay, quantitative reverse-transcription PCR, and Western blot we identified
the potential mechanism.
Results: We
confirmed for the first time that the expression of ZNF277 is significantly
increased in OC tissues and cell lines and that it is closely associated with
the adverse clinical features of OC patients. We demonstrated that
overexpression of ZNF277 potentiated the proliferation, migration, and invasion
of SKOV3 and OVCAR3 loss-of-function experiments showed that the silencing of
ZNF277 reduced the proliferation, migration, and invasion of OC cells.
Mechanistically, using quantitative chromatin immunoprecipitation assay,
luciferase reporter assay, quantitative reverse-transcription PCR, and Western
blot we identified that PTEN was a direct downstream target for ZNF277. PTEN
expression antagonized the tumor-promoting function of ZNF277.
Conclusion: Taken
together, the results of the current study demonstrated that ZNF277 exerted a
promoting role in the progression of OC and might act as a promising biomarker
and therapeutic target for OC patients.
Keywords: ZNF277,
ovarian cancer, proliferation, migration, invasion, PTEN