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Authors Ai J, Gong C, Wu J, Gao J, Liu W, Liao W, Wu L
Received 10 December 2018
Accepted for publication 28 March 2019
Published 23 April 2019 Volume 2019:11 Pages 3455—3467
DOI https://doi.org/10.2147/CMAR.S197716
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Andrew Yee
Peer reviewer comments 2
Editor who approved publication: Professor Nakshatri
Purpose: Numerous
studies have shown that the expression of microRNA-181c (miR-181c) is inhibited
in various cancers, which suggests that it has a cancer suppressive effect. In
the current study, we evaluated the regulation and characteristics of miR-181c
in human hepatocellular carcinoma (HCC).
Materials and methods: Samples
of tumor tissues and adjacent non-tumor tissues were collected from 52 patients
with HCC, and expression levels of miR-181c in these samples were investigated
via quantitative real-time polymerase chain reaction. HCC cell migration and
invasion were investigated via wound healing assays and transwell assays. HCC
cell apoptosis rates were assessed via flow cytometry, and HCC proliferation
was assessed via 5-ethynyl-20-deoxyuridine assays. In vivo tumors were
initiated by subcutaneously inoculating HCC cells into nude mice. And various
biomarkers were investigated via western blotting.
Results: In
microarray datasets and tumor tissues, significant downregulation of miR-181c
was apparent compared with non-tumorous adjacent tissues. Expression of
miR-181c in HCC cells was also significantly lower than it was in normal human
liver cells. miR-181c regulated the migration, invasion, apoptosis, and
proliferation of HCC cell lines in vitro, and tumor development in vivo.
Observations also suggest that miR-181c regulates NCAPG in HCC cells, and its
expression affects cellular invasion, migration, proliferation, and apoptosis.
There was a negative correlation between miR-181c expression and NCAPG in HCC
tissue samples.
Conclusion: miR-181c
exhibits tumor-suppression via the regulation of NCAPG levels.
Keywords: miR‑181c,
NCAPG, hepatocellular carcinoma, biological function