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通过液体激光消融对制备体内 MRI 造影剂氧化钆 @ 二氧化硅纳米颗粒的毒性评价

 

Authors Tian XM, Yang FW, Yang C, Peng Y, Chen DH, Zhu JX, He FP, Li L, Chen XM

Published Date August 2014 Volume 2014:9(1) Pages 4043—4053

DOI http://dx.doi.org/10.2147/IJN.S66164

Received 14 April 2014, Accepted 15 May 2014, Published 21 August 2014

Abstract: Poor toxicity characterization is one obstacle to the clinical deployment of Gd2O3@SiO2 core-shell nanoparticles (Gd-NPs) for use as magnetic resonance (MR) imaging contrast agents. To date, there is no systematic toxicity data available for Gd-NPs prepared by laser ablation in liquid. In this article, we systematically studied the Gd-NPs’ cytotoxicity, apoptosis in vitro, immunotoxicity, blood circulation half-life, biodistribution and excretion in vivo, as well as pharmacodynamics. The results show the toxicity, and in vivo MR data show that these NPs are a good contrast agent for preclinical applications. No significant differences were found in cell viability, apoptosis, and immunotoxicity between our Gd-NPs and Gd in a DTPA (diethylenetriaminepentaacetic acid) chelator. Biodistribution data reveal a greater accumulation of the Gd-NPs in the liver, spleen, lung, and tumor than in the kidney, heart, and brain. Approximately 50% of the Gd is excreted via the hepatobiliary system within 4 weeks. Furthermore, dynamic contrast-enhanced T1-weighted MR images of xenografted murine tumors were obtained after intravenous administration of the Gd-NPs. Collectively, the single step preparation of Gd-NPs by laser ablation in liquid produces particles with satisfactory cytotoxicity, minimal immunotoxicity, and efficient MR contrast. This may lead to their utility as molecular imaging contrast agents in MR imaging for cancer diagnosis.
Keywords: gadolinium, molecular imaging, cytotoxicity, immunotoxicity







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