论文已发表
注册即可获取德孚的最新动态
IF 收录期刊
Authors Lin J, Jia C, Wang Y, Jiang S, Jia Z, Chen N, Sheng S, Li S, Jiang L, Xu H, Zhou K, Chen Y
Received 21 November 2018
Accepted for publication 23 March 2019
Published 1 May 2019 Volume 2019:13 Pages 1461—1472
DOI https://doi.org/10.2147/DDDT.S195479
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Cristina Weinberg
Peer reviewer comments 4
Editor who approved publication: Prof. Dr. Cristiana Tanase
Background: Random
skin flap is frequently used in plastic and reconstructive surgery, but its
distal part often occurs ischemia and necrosis. Pravastatin (Prava) with
bioactivities of pro-angiogenesis, anti-apoptosis and anti-oxidative stress,
may be beneficial for flap survival.
Materials and methods: A
modified McFarlane flap model was performed in Sprague-Dawley rats. The animals
were divided into the Control and Prava groups and treated as follows: the
Prava group was injected intraperitoneally with 2 mg/kg Prava for consecutive 7
days, and the Control group received an equal volume of vehicle daily. On day
7, the necrosis skin flaps were observed, while visualization of blood flow
below the tissue surface was performed by Laser Doppler blood flow imaging
(LDBFI). Then animals were euthanized, and levels of angiogenesis, apoptosis
and oxidative stress were analyzed.
Results: Prava
decreased necrosis and edema of skin flaps compared with the Control group,
with more blood flow in the flap under LDBFI. Prava treatment increased the
mean vessels density, elevated the expression levels of angiogenic proteins
(matrix metallopeptidase 9, vascular endothelial growth factor, Cadherin5) and
antioxidant proteins (superoxide dismutase 1 (SOD1), endothelial nitric oxide
synthase, heme oxygenase), and decreased the expression of apoptotic factors
(BAX, CYC, Caspase3). In addition, malondialdehyde content was reduced, and
glutathione level and SOD activity were increased in the skin flaps after
treatment with Prava.
Conclusion: Prava
promotes survival of random skin flap through induction of angiogenesis, and
inhibition of apoptosis and oxidative stress.
Keywords: Pravastatin,
random skin flap, angiogenesis, oxidative stress, apoptosis