论文已发表
注册即可获取德孚的最新动态
IF 收录期刊
Authors Zhang M, Duan W, Sun W
Received 7 December 2018
Accepted for publication 28 March 2019
Published 2 May 2019 Volume 2019:12 Pages 3349—3360
DOI https://doi.org/10.2147/OTT.S197433
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Cristina Weinberg
Peer reviewer comments 2
Editor who approved publication: Dr Federico Perche
Objective: Colorectal
cancer (CRC) is a leading cause of cancer-related deaths worldwide. Small
nucleolar RNA host gene 6 (SNHG6) was reported to function as an oncogene in a
number of cancers. Here, we aimed to further explore the roles and molecular
mechanism of SNHG6 in CRC metastasis.
Methods: The
expression levels of SNHG6, miR-26a, and enhancer of zeste homolog 2 (EZH2)
mRNA were assessed by quantification real-time PCR in CRC tissues and cell
lines. Western blot analysis was performed to determine the levels of
E-cadherin, Snail, Vimentin, N-cadherin, and EZH2. Cell migration and invasion
capacities were detected by transwell assay. Dual-luciferase reporter assay or
RNA Immunoprecipitation assay was employed to verify the interaction between
SNHG6 and miR-26a, or EZH2 and miR-26a.
Results: Our data
indicated that SNHG6 and EZH2 mRNA were upregulated, and miR-26a was
downregulated in CRC tissues and cell lines. SNHG6 knockdown suppressed the
migration, invasion, and epithelial-mesenchymal transition (EMT) of CRC cells.
Moreover, SNHG6 binded to miR-26a and repressed miR-26a expression. EZH2 was a
direct target of miR-26a, and it was regulated by SNHG6/miR-26a. MiR-26a
inhibitor undermined the effect of SNHG6 knockdown on cell migration, invasion,
and EMT. Additionally, EZH2 antagonized the effect of miR-26a on cell migration,
invasion, and EMT in CRC cells.
Conclusion: SNHG6
knockdown suppressed cell migration, invasion, and EMT at least partly by
sponging miR-26a and regulating EZH2 expression in CRC cells, providing a
strategy for blocking CRC metastasis.
Keywords: small nucleolar
RNA host gene 6 (SNHG6), miR-26a, enhancer of zeste homolog 2 (EZH2),
epithelial-mesenchymal transition (EMT)