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TfR1 的下调通过 JAK/STAT 途径促进结直肠癌的进展
Authors Cui C, Cheng X, Yan L, Ding H, Guan X, Zhang W, Tian X, Hao C
Received 20 December 2018
Accepted for publication 20 April 2019
Published 9 July 2019 Volume 2019:11 Pages 6323—6341
DOI https://doi.org/10.2147/CMAR.S198911
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Amy Norman
Peer reviewer comments 2
Editor who approved publication: Professor Nakshatri
Background: Colorectal cancer (CRC) is one of the most prevalent gastrointestinal malignancies. The incidence of CRC has been rapidly increasing in China. Transferrin receptor 1 (TfR1) is a key regulator of cellular iron homeostasis. Several studies have demonstrated TfR1 overexpression in a variety of human tumors, but the association between TfR1 and CRC remains unclear.
Methods: TfR1 expression was evaluated in six CRC cell lines and tumor tissues. A total of 201 CRC patients were included for immunohistochemistry and 19 pairs of frozen tissues were used for real-time PCR. Cell proliferation, cell cycle, cell migration and invasion, and in vivo carcinogenesis were tested after downregulation of TfR1 by lentivirus. Protein microarray and Western blot analyses were used to explore the underlying mechanisms of TfR1 in CRC.
Results: TfR1 expression was higher in CRC tissues than in normal tissues (57.2% vs 22.9%, P <0.001). TfR1 expression was obviously higher in CRC tissues with well differentiation (P =0.008), no lymph node metastasis (P =0.002), no distant metastasis (P =0.006), no vascular invasion (P <0.001) and early TNM stage (P =0.013). CRC patients with TfR1-positive expression had a better survival than those with TfR1-negative expression (P =0.044). Downregulation of TfR1 expression inhibited cell proliferation, promoted cells from G1 phase to S phase and facilitated cell migration and invasion. Knockdown of TfR1 also suppressed tumor growth in BALB/C-nu mice. Protein microarray and Western blot analyses showed that the Janus protein tyrosine kinase/signal transducer and activator of transcription pathway was activated along with downregulation of TfR1 expression.
Conclusion: Though TfR1 was overexpressed in colorectal cancer tissues, there was evidence that downregulation of TfR1 could promote cancer progression.
Keywords: colorectal cancer, transferrin receptor 1, tumor progression, JAK/STAT pathway