Background: As one of the most widely produced engineered nanomaterials, titanium dioxide nanoparticles (nano-TiO₂) are used in biomedicine and healthcare products, and as implant scaffolds; therefore, the toxic mechanism of nano-TiO₂ has been extensively investigated with a view to guiding application. Three-dimensional (3D) spheroid models can simplify the complex physiological environment and mimic the in vivo architecture of tissues, which is optimal for the assessment of nano-TiO₂ toxicity under ultraviolet A (UVA) irradiation.
Methods and Results: In the present study, the toxicity of nano-TiO₂ under UVA irradiation was investigated in 3D H22 spheroids cultured in fibrin gels. A significant reduction of approximately 25% in spheroid diameter was observed following treatment with 100 μg/mL nano-TiO₂ under UVA irradiation after seven days of culture. Nano-TiO₂ under UVA irradiation triggered the initiation of the TGF-β/Smad signaling pathway, increasing the expression levels of TGF-β 1, Smad3, Cdkn1a, and Cdkn2b at both the mRNA and protein level, which resulted in cell cycle arrest in the G1 phase. In addition, nano-TiO₂ under UVA irradiation also triggered the production of reactive oxygen species (ROS), which were shown to be involved in cell cycle regulation and the induction of TGF-β 1 expression.
Conclusion: Nano-TiO₂ under UVA irradiation induced cell cycle arrest in the G1 phase and the formation of smaller spheroids, which were associated with TGF-β/Smad signaling pathway activation and ROS generation. These results reveal the toxic mechanism of nano-TiO₂ under UVA irradiation, providing the possibility for 3D spheroid models to be used in nanotoxicology studies.
Keywords: nano-TiO₂, cell cycle arrest, TGF-β signaling pathway, reactive oxygen species, 3D spheroid culture