Purpose: This study aimed to elucidate the biological function and upstream regulatory mechanism of CELSR1 in glioma.
Materials and Methods: We evaluated the expression of CELSR1 in glioma by TCGA_GEPIA tool, RT-qPCR, and Western blot assays. CCK-8, wound healing, and transwell invasion assays were, respectively, performed to detect the effect of CELSR1 on cell proliferation, migration, and invasion. The upstream regulatory miRNAs of CELSR1 were predicted by TargetScan and validated by luciferase activity reporter assay.
Results: CELSR1 is overexpressed in glioma (P < 0.05). CELSR1 promoted glioma cell proliferation, migration and invasion (P < 0.01). CELSR1 was a direct target of miR-199a-5p. miR199a-5p mimics significantly inhibited CELSR1 mRNA and protein expression (P < 0.01). miR199a-5p mimics reversed the effects of CELSR1 on glioma cell behaviors (P < 0.01).
Conclusion: CELSR1 acts as an oncogene promoting glioma cell proliferation, migration, and invasion, which is regulated by miR199a-5p.
Keywords: CELSR1, miR-199a-5p, glioma, proliferation, migration, invasion