Objective: Aneurysmal subarachnoid hemorrhage (aSAH) is a severe form of stroke characterized by high rates of mortality and disability. Identifying circulating biomarkers is helpful to improve outcomes. In this study, for the first time, we identify interleukin-6 (IL-6) in cerebrospinal fluid (CSF) small extracellular vesicles (sEVs) as potential biomarkers for prognosis of aSAH.
Methods: We extracted small extracellular vesicles from the CSF of 103 aSAH patients and 40 healthy controls in a prospective observational study. Subsequently, we measured IL-6_sEVs levels using an enzyme-linked immunosorbent assay. Results were statistically analyzed to determine the function of IL-6_sEVs for disease monitoring of aSAH.
Results: CSF IL-6 _sEVs showed distinct pattern differences between healthy controls and aSAH patients. The concentration of IL-6_sEVs in CSF is significantly correlated with the severity of aSAH patients. The areas under the receiver operating characteristic curves of IL-6_sEVs for identifying severe aSAH patient from aSAH patients were 0.900. After multivariate logistic regression analysis, IL-6_sEVs were associated with neurological outcome at 1 year. IL-6_sEVs levels were greater and positively associated with disease processes and outcome.
Conclusion: There is a neuroinflammatory cascade in aSAH patients. IL-6_sEVs in CSF may be a biomarker for the progression of aSAH.
Keywords: subarachnoid hemorrhage, cerebrospinal fluid, small extracellular vesicles, interleukin-6, biomarker