Background: Mycobacterium fortuitum  is a rapidly growing non-tuberculous mycobacterium (NTM) with weak pathogenicity. Here, we present a rare case of disseminated M. fortuitum  and Talaromyces marneffei  coinfection in a human immunodeficiency virus (HIV) negative patient.
Case Presentation: A 28-year-old female was admitted to our hospital due to 2 months of swelling of lymph nodes on the right side of her cervix, accompanied by repeated low fever for more than 1 month. Biopsy of the right cervical lymph node and endobronchial ultrasound-guided transbronchial fine needle aspiration (EBUS-TBNA) both suggested granulomatous inflammation. The bacterial culture and mycobacteria examination of the lesion as well as HIV antibody test were all negative. Disseminated T. marneffei  infection was diagnosed by the quantitative polymerase chain reaction (qPCR) results from the blood showing 1798 copies/ul. In the meantime, treatment with amphotericin B combined with cefoxitin was administered for suspected NTM infection. However, the once-dropped fever recurred and the lymph nodes continued to swell. Metagenomics next-generation sequencing (mNGS) detection of the lymph nodes indicated M. fortuitum . After combination treatment with amphotericin B, voriconazole, linazolamide, and imipenem, the patient’s body temperature returned to normal, the lymph node swelling was gradually reduced, and the lung lesion was absorbed.
Conclusion: We report the first case of an HIV-negative patient diagnosed with disseminated M. fortuitum  and T. marneffei  coinfection with nonspecific clinical manifestation, in order to heighten awareness of these infections.
Keywords: Mycobacterium fortuitum , Talaromyces marneffei , coinfection, non-HIV