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已发表论文
TNF/NF-κB 信号通路参与通络汤对盆腔炎性疾病后遗症大鼠模型的作用机制
Authors Liu B, Yang F, Pu X, Yu J, Wang Q
Received 21 January 2025
Accepted for publication 30 August 2025
Published 17 September 2025 Volume 2025:18 Pages 12949—12960
DOI https://doi.org/10.2147/JIR.S518734
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Professor Ning Quan
Baoqin Liu,1 Fang Yang,1 Xiaoqi Pu,2 Junjie Yu,1 Qing Wang1
1Traditional Chinese Medicine Gynecology, China-Japan Friendship Hospital, Beijing, People’s Republic of China; 2Diagnostic Radiology, China-Japan Friendship Hospital, Beijing, People’s Republic of China
Correspondence: Qing Wang, Traditional Chinese Medicine Gynecology, China-Japan Friendship Hospital, No. 2 Yinghua East Street, Chaoyang District, Beijing, 100029, People’s Republic of China, Email bqin_2919@163.com
Purpose: Traditional Chinese medicine can produce strong therapeutic activities for sequelae of pelvic inflammatory disease (SPID). This study aimed to investigate the efficacy and protective mechanisms of Tongluo Decoction (TLD) in the treatment of SPID.
Patients and Methods: The damage-protective and inflammation-inhibitory effects of TLD on SPID were investigated in rat models. The possible targets of TLD against SPID were predicted using network pharmacology. The hub targets were tested in SPID rat models. Rescue experiments were conducted in human endometrial epithelial cells (HEECs). Detection of necroptosis was used the flow cytometry. ELISA was used for the quantification of inflammatory cytokines. Protein levels were detected using a Western blot assay.
Results: TLD had a protective effect against uterus tissue damage caused during SPID. TLD inhibited the levels of adhesion cytokines (VEGF and TGF-β 1), the tight junction proteins (ZO-1 and occludin), and inflammatory cytokines (TNF-α and IL-6). TNF, IKBKB, and NFKB1 were predicted as hub targets for TLD against SPID. TLD inhibited necroptosis of HEECs via TNF, IKBKB, and NFKB1. TLD inhibited the ratio of CD86+ M1 macrophages after differentiation induction of THP-1.
Conclusion: The therapeutic effects of TLD for SPID may be the result of a dual action on inflammation and necroptosis, via TNF, IKBKB, and NFKB1.
Keywords: herb, formula, uterus, inflammation, necroptosis
1Traditional Chinese Medicine Gynecology, China-Japan Friendship Hospital, Beijing, People’s Republic of China; 2Diagnostic Radiology, China-Japan Friendship Hospital, Beijing, People’s Republic of China
Correspondence: Qing Wang, Traditional Chinese Medicine Gynecology, China-Japan Friendship Hospital, No. 2 Yinghua East Street, Chaoyang District, Beijing, 100029, People’s Republic of China, Email bqin_2919@163.com
Purpose: Traditional Chinese medicine can produce strong therapeutic activities for sequelae of pelvic inflammatory disease (SPID). This study aimed to investigate the efficacy and protective mechanisms of Tongluo Decoction (TLD) in the treatment of SPID.
Patients and Methods: The damage-protective and inflammation-inhibitory effects of TLD on SPID were investigated in rat models. The possible targets of TLD against SPID were predicted using network pharmacology. The hub targets were tested in SPID rat models. Rescue experiments were conducted in human endometrial epithelial cells (HEECs). Detection of necroptosis was used the flow cytometry. ELISA was used for the quantification of inflammatory cytokines. Protein levels were detected using a Western blot assay.
Results: TLD had a protective effect against uterus tissue damage caused during SPID. TLD inhibited the levels of adhesion cytokines (VEGF and TGF-β 1), the tight junction proteins (ZO-1 and occludin), and inflammatory cytokines (TNF-α and IL-6). TNF, IKBKB, and NFKB1 were predicted as hub targets for TLD against SPID. TLD inhibited necroptosis of HEECs via TNF, IKBKB, and NFKB1. TLD inhibited the ratio of CD86+ M1 macrophages after differentiation induction of THP-1.
Conclusion: The therapeutic effects of TLD for SPID may be the result of a dual action on inflammation and necroptosis, via TNF, IKBKB, and NFKB1.
Keywords: herb, formula, uterus, inflammation, necroptosis