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已发表论文
WEE2 基因多态性与接受卵胞浆内单精子注射女性受精失败的关联
Authors Al-Gawwam ZHM , Abdulhussein RH, Abed HS, Wei W, Cai M
Received 6 June 2025
Accepted for publication 27 August 2025
Published 15 September 2025 Volume 2025:17 Pages 3045—3053
DOI https://doi.org/10.2147/IJWH.S545145
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Matteo Frigerio
Zahraa Hamid Mohan Al-Gawwam,1 Rasha Hamzah Abdulhussein,2 Hala Saad Abed,3 Wengong Wei,4 Meiling Cai4
1Department of Anatomy, Hammurabi College of Medicine, University of Babylon, Hillah, Babylon, 51002, Iraq; 2Department of Medical Physiology, Hammurabi College of Medicine, University of Babylon, Hillah, Babylon, 51002, Iraq; 3Department of Pharmacology, Faculty of Medicine, University of Babylon, Hillah, Babylon, 51002, Iraq; 4Department of Obstetrics and Gynecology, Qingpu Branch, Zhongshan Hospital, Fudan University, Shanghai, 201700, People’s Republic of China
Correspondence: Meiling Cai, Department of Obstetrics and Gynecology, Qingpu Branch, Zhongshan Hospital, Fudan University, Shanghai, 201700, People’s Republic of China, Tel +8618116016012, Email cai.meiling@qphospital.com
Background: Infertility and pregnancy loss are major concerns in reproductive medicine, often linked to genetic factors affecting oocyte maturation. The WEE2 gene, which encodes an oocyte-specific kinase critical for meiosis, plays a vital role in fertilization. Variants in WEE2 have been implicated in oocyte maturation arrest and total fertilisation failure (TFF), particularly in women undergoing assisted reproductive technologies. This study aimed to investigate the association between WEE2 gene polymorphism and fertilization failure in women undergoing intracytoplasmic sperm injection (ICSI).
Materials and Methods: This prospective comparative study enrolled 137 infertile women undergoing ICSI procedures. Peripheral blood samples were collected from all participants for genetic analysis. Genotyping of the WEE2 gene variant rs1476640 was performed using a polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) assay, which was specifically designed and optimised for this study.
Results: WEE2 gene polymorphism (rs1476640) showed a significant association with fertilization outcomes (p < 0.0001). The T allele was protective, while the C allele increased the risk of fertilization failure (OR = 9.06; 95% CI: 3.27– 25.14). Significant differences were also observed in infertility duration, oocyte count, and endometrial thickness between pregnant and non-pregnant women (p < 0.05), but not in age or BMI.
Conclusion: The WEE2 rs1476640 polymorphism is significantly associated with fertilization failure in ICSI-treated women. Screening for this variant may help identify patients at risk and support personalised infertility treatment strategies.
Keywords: infertility, oogenesis, gene polymorphism of WEE2, fertilization failure
1Department of Anatomy, Hammurabi College of Medicine, University of Babylon, Hillah, Babylon, 51002, Iraq; 2Department of Medical Physiology, Hammurabi College of Medicine, University of Babylon, Hillah, Babylon, 51002, Iraq; 3Department of Pharmacology, Faculty of Medicine, University of Babylon, Hillah, Babylon, 51002, Iraq; 4Department of Obstetrics and Gynecology, Qingpu Branch, Zhongshan Hospital, Fudan University, Shanghai, 201700, People’s Republic of China
Correspondence: Meiling Cai, Department of Obstetrics and Gynecology, Qingpu Branch, Zhongshan Hospital, Fudan University, Shanghai, 201700, People’s Republic of China, Tel +8618116016012, Email cai.meiling@qphospital.com
Background: Infertility and pregnancy loss are major concerns in reproductive medicine, often linked to genetic factors affecting oocyte maturation. The WEE2 gene, which encodes an oocyte-specific kinase critical for meiosis, plays a vital role in fertilization. Variants in WEE2 have been implicated in oocyte maturation arrest and total fertilisation failure (TFF), particularly in women undergoing assisted reproductive technologies. This study aimed to investigate the association between WEE2 gene polymorphism and fertilization failure in women undergoing intracytoplasmic sperm injection (ICSI).
Materials and Methods: This prospective comparative study enrolled 137 infertile women undergoing ICSI procedures. Peripheral blood samples were collected from all participants for genetic analysis. Genotyping of the WEE2 gene variant rs1476640 was performed using a polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) assay, which was specifically designed and optimised for this study.
Results: WEE2 gene polymorphism (rs1476640) showed a significant association with fertilization outcomes (p < 0.0001). The T allele was protective, while the C allele increased the risk of fertilization failure (OR = 9.06; 95% CI: 3.27– 25.14). Significant differences were also observed in infertility duration, oocyte count, and endometrial thickness between pregnant and non-pregnant women (p < 0.05), but not in age or BMI.
Conclusion: The WEE2 rs1476640 polymorphism is significantly associated with fertilization failure in ICSI-treated women. Screening for this variant may help identify patients at risk and support personalised infertility treatment strategies.
Keywords: infertility, oogenesis, gene polymorphism of WEE2, fertilization failure